Second harmonic imaging of chloroplasts using the two-photon laser scanning microscope

Reshak, Ali Hussin, Sarafis, Vassilios and Heintzmann, Rainer (2009) Second harmonic imaging of chloroplasts using the two-photon laser scanning microscope. Micron, 40 3: 378-385. doi:10.1016/j.micron.2008.09.007


Author Reshak, Ali Hussin
Sarafis, Vassilios
Heintzmann, Rainer
Title Second harmonic imaging of chloroplasts using the two-photon laser scanning microscope
Journal name Micron   Check publisher's open access policy
ISSN 0968-4328
Publication date 2009-04-01
Year available 2008
Sub-type Article (original research)
DOI 10.1016/j.micron.2008.09.007
Open Access Status DOI
Volume 40
Issue 3
Start page 378
End page 385
Total pages 8
Place of publication U K
Publisher Pergamon
Language eng
Subject C1
970106 Expanding Knowledge in the Biological Sciences
0699 Other Biological Sciences
Abstract We employed several complementary cell image analytical methods including ImageStream system (ISS analysis) andmolecular approaches to identify and purify from adult murine organs a population of very small embryonic like stem cells (VSELs). These cells are (i) small in size, (ii) possess high cytoplasmic/ nuclear ratio, (iii) contain primitive unorganized euchromatin, (iv) in mice are found among Sca-1+ Lin CD45 cells and in humans among CD133+ CXCR4+ CD34+ Lin CD45 cells and (v) express embryonic markers such as Oct-4 protein in nuclei and SSEA antigens on the surface. In mice the highest number of these cells resides in brain, kidney, pancreas and bone marrow. Data from our laboratory indicate that VSELs are most likely a population of germ line/epiblast-derived pluripotent stem cells, that is deposited during organogenesis in developing tissues as a source of tissue committed stem cells and that the number of these cells decreases with the age. We believe that VSELs could be harnessed as a source of pluripotent stem cells for regenerative medicine.
Q-Index Code C1
Q-Index Status Provisional Code

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biological Sciences Publications
 
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Created: Sat, 24 Apr 2010, 00:30:14 EST by Gail Walter on behalf of School of Biological Sciences