Real-time PCR genotyping and frequency of the myostatin F94L mutation in beef cattle breeds

Vankan, D. M., Waine, D. R. and Fortes, M. R. S. (2010) Real-time PCR genotyping and frequency of the myostatin F94L mutation in beef cattle breeds. Animal, 4 4: 530-534. doi:10.1017/S175173110999139X

Author Vankan, D. M.
Waine, D. R.
Fortes, M. R. S.
Title Real-time PCR genotyping and frequency of the myostatin F94L mutation in beef cattle breeds
Journal name Animal   Check publisher's open access policy
ISSN 1751-7311
Publication date 2010-04-01
Year available 2009
Sub-type Article (original research)
DOI 10.1017/S175173110999139X
Volume 4
Issue 4
Start page 530
End page 534
Total pages 5
Place of publication Cambridge, United Kingdom
Publisher Cambridge University Press
Language eng
Abstract This research developed two real-time PCR assays, employing high-resolution melt and allele-specific analysis to accurately genotype the F94L mutation in cattle. This mutation (g.433C > A) in the growth differentiation factor 8 or myostatin gene has recently been shown to be functionally associated with increased muscle mass and carcass yield in cattle. The F94L mutation is not, like other myostatin mutations, associated with reduced fertility and dystocia. It is therefore a candidate for introgression into other breeds to improve retail beef yield and the development of a simple and accurate test to genotype this specific mutation is warranted. Variations in the efficiency of enzyme cleavage compromised the accuracy of genotyping by published methods, potentially resulting in an overestimation of the frequency of the mutant allele. The frequency of the F94L mutation was determined by real-time PCR in 1140 animals from 15 breeds of cattle in Australia. The mutation was present in Simmental (0.8%), Piedmontese (2%), Droughtmaster (4%) and Limousin (94.2%) but not found in Salers, Angus, Poll Hereford, Hereford, Gelbvieh, Charolais, Jersey, Brahman, Holstein, Shorthorn or Maine Anjou. The low prevalence of F94L in all beef breeds except Limousin indicates the significant potential for this mutation to improve retail yield in Australian beef cattle. © 2009 The Animal Consortium.
Keyword Beef cattle
Marker-assisted selection
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online 27 November 2009

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Veterinary Science Publications
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Created: Sun, 11 Apr 2010, 10:10:38 EST