Controlled preparation of layered double hydroxide nanoparticles and their application as gene delivery vehicles

Ladewig, K., Niebert, M., Xu, Z.P., Gray, P.P. and Lu, G.Q. (2010) Controlled preparation of layered double hydroxide nanoparticles and their application as gene delivery vehicles. Applied Clay Science, 48 1-2: 280-289. doi:10.1016/j.clay.2009.11.032

Author Ladewig, K.
Niebert, M.
Xu, Z.P.
Gray, P.P.
Lu, G.Q.
Title Controlled preparation of layered double hydroxide nanoparticles and their application as gene delivery vehicles
Journal name Applied Clay Science   Check publisher's open access policy
ISSN 0169-1317
Publication date 2010-03-01
Year available 2009
Sub-type Article (original research)
DOI 10.1016/j.clay.2009.11.032
Open Access Status Not Open Access
Volume 48
Issue 1-2
Start page 280
End page 289
Total pages 10
Editor G.J.Churchman
Place of publication Netherlands
Publisher Elsevier BV
Language eng
Subject C1
Abstract Layered double hydroxides (LDHs) have been known for many decades as catalyst and ceramic precursors, traps for anionic pollutants, catalysts, and additives for polymers, but they recently attracted attention as potential nano-sized carriers for therapeutic/bio-active molecules and genes. Among the many different nanoparticles that have been shown to facilitate gene and/or drug delivery, LDH nanoparticles are particularly well suited for this purpose due to their many desirable properties. In this research Mg2Al(OH)(6)NO3 LDH nanoparticles of varying lateral sizes were synthesized by altering the synthesis conditions. The synthesis conditions particularly influencing the particle size distribution of the LDH suspensions are (a) the temperature during the co-precipitation step and (b) the duration and the temperature of the hydrothermal treatment The association of these nanoparticles with plasmid DNA was studied and it was established that-in contrast to previously published reports-for the plasmid sizes used no significant intercalation occurs. The plasmids wrap around individual particles instead and aggregation of particles is observed. However, due to the observed strong interaction between LDH nanoparticles and DNA, the particles were nonetheless evaluated as transfection agents for mammalian cells. Considerable transfection efficiencies when transfecting adherent cell lines (i.e., HEK293T, NIH 3T3, COS-7, and CHO-K1) were observed, while the transfection of suspension CHO-S cells remained unsuccessful. This is attributed to the formation of aggregates upon DNA-LDH complex formation which settle on top of adherent cells but due to the constant agitation of suspension cultures not on the surface of e.g., CHO-S cells. (C) 2009 Elsevier B.V. All rights reserved.
Keyword Layer double hydroxides
Gene delivery
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
Australian Institute for Bioengineering and Nanotechnology Publications
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Citation counts: TR Web of Science Citation Count  Cited 62 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 66 times in Scopus Article | Citations
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Created: Sun, 11 Apr 2010, 10:10:18 EST