Functional protective role for mucin glycosylated repetitive domains

Loomes, Kerry M., Senior, Hugh E., West, Paula M. and Roberton, Anthony M. (1999) Functional protective role for mucin glycosylated repetitive domains. European Journal of Biochemistry, 266 1: 105-111. doi:10.1046/j.1432-1327.1999.00824.x

Author Loomes, Kerry M.
Senior, Hugh E.
West, Paula M.
Roberton, Anthony M.
Title Functional protective role for mucin glycosylated repetitive domains
Journal name European Journal of Biochemistry   Check publisher's open access policy
ISSN 0014-2956
Publication date 1999-11-15
Year available 1999
Sub-type Article (original research)
DOI 10.1046/j.1432-1327.1999.00824.x
Open Access Status Not Open Access
Volume 266
Issue 1
Start page 105
End page 111
Total pages 7
Place of publication Oxford, United Kingdom
Publisher Wiley-Blackwell Publishing
Language eng
Subject 1101 Medical Biochemistry and Metabolomics
110307 Gastroenterology and Hepatology
Abstract Mucins carry out a number of protective roles, some of which are more easily studied than others. One mucin function is believed to be the protection of the mucosal epithelium against acidic and proteolytic damage in the stomach and intestines. In the present work, a portion of stomach mucin tandem repeat sequence (Muc6) was joined to the catalytic domain of a reporter enzyme [human milk cholesterol esterase (CE)] to determine whether the former can protect the latter protein from damage. This Muc6 domain replaced a unique series of glycosylated C-terminal repeats normally present in CE. The chimeric protein (CE/Muc6) was expressed in two different cell lines and its properties compared to recombinant full-length CE and a truncated version of CE which contained only the catalytic domain (CE/trunc). Results showed that both CE and CE/Muc6 were resistant to denaturation by acid and to proteolysis by pepsin at low pH values or by pancreatic proteases compared to CE/trunc. Thus, a stomach Muc6 domain is sufficient to confer stability on the CE catalytic domain, demonstrating a protective effect by a glycosylated mucin sequence.
Keyword Mucin
Cholesterol esterase
pH stability
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown
Additional Notes Continued by FEBS journal

Document type: Journal Article
Sub-type: Article (original research)
Collection: Discipline of General Practice Publications
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Created: Fri, 09 Apr 2010, 02:18:05 EST by Dr Hugh Senior on behalf of School of Medicine