High frequency of fetal cells within a primitive stem cell population in maternal blood

Mikhail, Magued A., M’Hamdi, Hanane, Welsh, Jonathan, Levicar, Natasa, Marley, Stephen B., Nicholls, Joanna P., Habib, Nagy A., Louis, Louay S., Fisk, Nicholas M. and Gordon, Myrtle Y. (2008) High frequency of fetal cells within a primitive stem cell population in maternal blood. Human Reproduction, 23 4: 928-933. doi:10.1093/humrep/dem417

Author Mikhail, Magued A.
M’Hamdi, Hanane
Welsh, Jonathan
Levicar, Natasa
Marley, Stephen B.
Nicholls, Joanna P.
Habib, Nagy A.
Louis, Louay S.
Fisk, Nicholas M.
Gordon, Myrtle Y.
Title High frequency of fetal cells within a primitive stem cell population in maternal blood
Journal name Human Reproduction   Check publisher's open access policy
ISSN 1460-2350
Publication date 2008-04-01
Sub-type Article (original research)
DOI 10.1093/humrep/dem417
Volume 23
Issue 4
Start page 928
End page 933
Total pages 6
Place of publication London, U.K.
Publisher Oxford University Press
Language eng
Subject 1103 Clinical Sciences
1114 Paediatrics and Reproductive Medicine
Formatted abstract

BACKGROUND: During pregnancy, fetal cells enter the maternal bloodstreamresulting in fetal cell microchimerism. The fetal cells persist in the mother for decades and colonize a variety of maternal organs. They are associated with maternal autoimmune diseases and may also participate in tissue repair. The identity of the microchimeric cells is not certain but they must be able to persist long-term and have potential for multitissue differentiation.
METHODS AND RESULTS: Here we tested the hypothesis that the fetal microchimeric cells are primitive stem cells, represented by CD34+ adherent cells, which have a wide potential for differentiation. We isolated these stem cells from the blood of pregnant females (n = 25) and detected fetal cells of the correct gender, using fluorescence in situ hybridization, in a high proportion (71% male fetuses and 90% female fetuses; false positive rate 11%, false negative rate 29%) of cases. By RT–PCR, we demonstrated that the cells express Oct-4, Nanog and Rex-1. No fetal cells were detected in the mononuclear or total CD34+ cell populations but high frequencies (mean 11.8%) of fetal cells were detected in the adherent CD34+ cell population.
CONCLUSIONS: These results identify adherent CD34+ stem cells as candidate fetal microchimeric cells, which are capable of sustaining the fetal cell population in the long term and have the ability to colonize multiple tissues and organs. 

Keyword CD34
Fetal stem cells
Q-Index Code C1
Q-Index Status Provisional Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Health and Rehabilitation Sciences Publications
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 25 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 31 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 06 Apr 2010, 22:47:13 EST by Ms May Balasaize on behalf of Faculty Of Health Sciences