Morphological changes in white matter astrocytes in response to hypoxia/ischemia in the neonatal pig

Sullivan, Susan M., Bjorkman, S. Tracey, Miller, Stephanie M., Colditz, Paul B. and Pow, David V. (2010) Morphological changes in white matter astrocytes in response to hypoxia/ischemia in the neonatal pig. Brain Research, 1319 164-174. doi:10.1016/j.brainres.2010.01.010

Author Sullivan, Susan M.
Bjorkman, S. Tracey
Miller, Stephanie M.
Colditz, Paul B.
Pow, David V.
Title Morphological changes in white matter astrocytes in response to hypoxia/ischemia in the neonatal pig
Journal name Brain Research   Check publisher's open access policy
ISSN 0006-8993
Publication date 2010-03-01
Sub-type Article (original research)
DOI 10.1016/j.brainres.2010.01.010
Open Access Status Not Open Access
Volume 1319
Start page 164
End page 174
Total pages 11
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Subject 1109 Neurosciences
Formatted abstract
White matter damage is a significant problem in the human pre-term baby. Damage to white matter is usually associated with injury or insults to babies born prematurely, typically before 32 weeks' gestation, however there is increasing evidence of both grey and white matter damage occurring after 32 weeks' gestation. Astrocytes play a vital role in white matter, regulating molecules such as glutamate in the extracellular space and preventing excitotoxic damage to neighbouring oligodendrocytes and axons. We have previously described dramatic changes in grey matter astrocytes in response to a hypoxic/ischemic (H/I) insult around the time of birth. In this study, we have used GFAP immunohistochemistry and Golgi–Kopsch staining to examine the morphology of white matter astrocytes in control neonatal pig brains, and in the brains of animals exposed to the same (perinatal) H/I insult. We demonstrate that the areal percentage of the section occupied by GFAP-immunoreactive processes and cell bodies is significantly decreased (by 46%, P < 0.0001) in subcortical white matter from H/I brains. This loss of GFAP was accompanied by alterations in astrocyte morphology and an overall decrease in the size (field of section occupied by an individual astrocyte) of white matter astrocytes from 649 μm2 to 426 μm2, as revealed by Golgi–Kopsch staining and image analysis. These data suggest that astrocytes may contribute to the pathology of white matter damage following an H/I insult around the time of birth, and suggest that astrocytes may offer a novel target for therapies to improve outcomes after H/I.
Copyright © 2010 Elsevier B.V. All rights reserved.

Keyword Brain injury
Golgi staining
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published under Research Reports.

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Official 2011 Collection
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Citation counts: TR Web of Science Citation Count  Cited 22 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 25 times in Scopus Article | Citations
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Created: Sun, 04 Apr 2010, 10:06:50 EST