Members of a large retroposon family are determinants of post-transcriptional gene expression in Leishmania

Bringaud, Frédéric, Müller, Michaela, Cerqueira, Gustavo Coutinho, Smith, Martin, Rochette, Annie, El-Sayed, Najib M. A., Papadopoulou, Barbara and Ghedin, Elodie (2007) Members of a large retroposon family are determinants of post-transcriptional gene expression in Leishmania. PLOS pathogens, 3 9: 1291-1307. doi:10.1371/journal.ppat.0030136


Author Bringaud, Frédéric
Müller, Michaela
Cerqueira, Gustavo Coutinho
Smith, Martin
Rochette, Annie
El-Sayed, Najib M. A.
Papadopoulou, Barbara
Ghedin, Elodie
Title Members of a large retroposon family are determinants of post-transcriptional gene expression in Leishmania
Journal name PLOS pathogens   Check publisher's open access policy
ISSN 1553-7366
1553-7374
Publication date 2007-09-01
Sub-type Article (original research)
DOI 10.1371/journal.ppat.0030136
Open Access Status DOI
Volume 3
Issue 9
Start page 1291
End page 1307
Total pages 17
Place of publication San Francisco, CA, U.S.A.
Publisher Public Library of Science
Language eng
Subject 0605 Microbiology
1107 Immunology
1108 Medical Microbiology
Formatted abstract
Trypanosomatids are unicellular protists that include the human pathogens Leishmania spp. (leishmaniasis), Trypanosoma brucei (sleeping sickness), and Trypanosoma cruzi (Chagas disease). Analysis of their recently completed genomes confirmed the presence of non-long-terminal repeat retrotransposons, also called retroposons. Using the 79-bp signature sequence common to all trypanosomatid retroposons as bait, we identified in the Leishmania major genome two new large families of small elements - LmSIDER1 (785 copies) and LmSIDER2 (1,073 copies) - that fulfill all the characteristics of extinct trypanosomatid retroposons. LmSIDERs are ∼70 times more abundant in L. major compared to T. brucei and are found almost exclusively within the 3′-untranslated regions (3′UTRs) of L. major mRNAs. We provide experimental evidence that LmSIDER2 act as mRNA instability elements and that LmSIDER2-containing mRNAs are generally expressed at lower levels compared to the non-LmSIDER2 mRNAs. The considerable expansion of LmSIDERs within 3′UTRs in an organism lacking transcriptional control and their role in regulating mRNA stability indicate that Leishmania have probably recycled these short retroposons to globally modulate the expression of a number of genes. To our knowledge, this is the first example in eukaryotes of the domestication and expansion of a family of mobile elements that have evolved to fulfill a critical cellular function.
© 2007 Bringaud et al.
Keyword 3' untranslated regions
Gene expression regulation
Protozoan genome
Leishmania
Leishmania major
Retroelements
Messenger Rna
Trypanosoma brucei brucei
Trypanosoma cruzi
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Chemistry and Molecular Biosciences
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 59 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 64 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Thu, 01 Apr 2010, 22:05:52 EST by June Temby on behalf of Faculty of Science