Over-expression of matrix metalloproteinase-10 is associated with vascular smooth muscle cell loss in Marfan Syndrome and bicuspid aortic valve aneurysm

Do Hong Lien, Nataatmadja Maria and West Malcolm (2008) Over-expression of matrix metalloproteinase-10 is associated with vascular smooth muscle cell loss in Marfan Syndrome and bicuspid aortic valve aneurysm. Heart, Lung and Circulation, 17 Supplement 3: S21-S21.

Author Do Hong Lien
Nataatmadja Maria
West Malcolm
Title Over-expression of matrix metalloproteinase-10 is associated with vascular smooth muscle cell loss in Marfan Syndrome and bicuspid aortic valve aneurysm
Journal name Heart, Lung and Circulation   Check publisher's open access policy
ISSN 1443-9506
Publication date 2008-07-15
Year available 2008
Sub-type Article (original research)
Open Access Status Not Open Access
Volume 17
Issue Supplement 3
Start page S21
End page S21
Total pages 1
Publisher Elsevier Inc.
Language eng
Subject E3
Abstract Thoracic aortic aneurysm associated with Marfan syndrome (MFS) or bicuspid aortic valve (BAV) is characterized by over-expression of matrix metalloproteinases (especially MMP-2 and MMP-9). Up-regulated MMP-10 has been linked to reduced cell–matrix interaction, cell detachment, dilation and rupture of blood vessels. We investigated MMP-10 expression in aortic aneurysm tissue and cultured VSMCs and its relation to VSMC apoptosis. Aortic tissue and cultured VSMCs were derived from subjects with MFS (five males, two females; 40 ± 22 years, mean ± S.D.) and BAV (five males, two females; 60 ± 16 years), and normal subjects (three males, six females; 44 ± 14 years). Caspase-3 was used as a marker of cells undergoing apoptosis. Specific staining of aortic tissue showed increased expression of MMP-10 in MFS and BAV (P < 0.05). Similar results were obtained in cultured VSMCs. In VSMCs characterized by cell shrinkage of cell and nuclear condensation there was enhanced staining of MMP-10. The same VSMCs showed caspase-3 labelling in alternate serial sections. The proportion of apoptotic VSMCs in aneurysm tissue was significantly increased in the aneurysm wall of patient groups compared to normal subjects (P < 0.05). Gelatin zymography showed no active form of MMP-10 and Western blot indicated the presence of the MMP-10 pro-form only with higher levels of expression in VSMC conditioned media from patients compared to normals (P < 0.05). The study suggests that expression of MMP-10 is related to progression of aortic wall aneurysm in MFS and BAV.
Q-Index Code EX
Q-Index Status Provisional Code

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
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Created: Wed, 31 Mar 2010, 21:13:22 EST by Hong Lien Do on behalf of Faculty Of Health Sciences