Enhanced tumor growth in the NaS1 sulfate transporter null mouse

Dawson, P A, Choyce, A, Chuang, C, Whitelock, J, Markovich, D and Leggatt, GR (2009) Enhanced tumor growth in the NaS1 sulfate transporter null mouse. Cancer Science, 101 2: 369-373. doi:10.1111/j.1349-7006.2009.01399.x

Author Dawson, P A
Choyce, A
Chuang, C
Whitelock, J
Markovich, D
Leggatt, GR
Title Enhanced tumor growth in the NaS1 sulfate transporter null mouse
Journal name Cancer Science   Check publisher's open access policy
ISSN 1347-9032
Publication date 2009-11-01
Year available 2009
Sub-type Article (original research)
DOI 10.1111/j.1349-7006.2009.01399.x
Open Access Status DOI
Volume 101
Issue 2
Start page 369
End page 373
Total pages 5
Editor Yusuke Nakamura
Place of publication Japan
Publisher Wiley-Blackwell Publishing Japan
Language eng
Subject C1
920102 Cancer and Related Disorders
1112 Oncology and Carcinogenesis
Abstract Sulfate plays an important role in maintaining normal structure and function of tissues, and its content is decreased in certain cancers including lung carcinoma. In this study, we investigated tumor growth in a mouse model of hyposulfatemia (Nas1(-/-)) and compared it to wild-type (Nas1(+/+)) mice. Lung epithelial tumor cells (TC-1 cell line) were injected subcutaneously into male Nas1(-/-) and Nas1(+/+) mice on a mixed 129Sv and C57BL/6 genetic background. Tumor sections were stained with anti-glycosaminoglycan antibodies to assess the distribution of proteoglycans and Gomori's trichrome to detect collagen. After 14 days, tumor weights were markedly increased (by similar to 12-fold) in Nas1(-/-) mice when compared with Nas1(+/+) mice. Histological analyses of tumors revealed increased (by approximate to 2.4-fold) vessel content, as well as markedly reduced collagen and immunoreactivity against glycosaminoglycan structural epitopes in the tumors from Nas1(-/-) mice. No significant differences were found for the growth of cultured TC-1 cells supplemented with Nas1(-/-) or Nas1(+/+) serum, as determined by H-3-thymidine incorporation, implying that the cell culture conditions may not reflect the in vivo situation of enhanced tumor growth. This study has revealed increased tumor growth and an altered extracellular tumor matrix in hyposulfatemic Nas1(-/-) mice. These findings highlight the importance of blood sulfate levels as a possible modulator of tumor growth, and could lead to future cancer studies in humans with altered sulfate homeostasis. (Cancer Sci 2010; 101: 369-373)
Q-Index Code C1
Q-Index Status Confirmed Code
Additional Notes published online Nov 2009

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
School of Biomedical Sciences Publications
UQ Diamantina Institute Publications
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Citation counts: TR Web of Science Citation Count  Cited 8 times in Thomson Reuters Web of Science Article | Citations
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Created: Tue, 30 Mar 2010, 21:24:56 EST by Bacsweet Kaur on behalf of School of Biomedical Sciences