Cloning of chimerical translocations as positive control for molecular genetic diagnosis of leukemia

Ustek, Duran, Sırma, Sema, Cakiris, Aris, Coşan, Fulya, Oku, Başar and Özbek, Uğur (2008) Cloning of chimerical translocations as positive control for molecular genetic diagnosis of leukemia. Turkish Journal of Hematology, 25 1: 20-23.

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Author Ustek, Duran
Sırma, Sema
Cakiris, Aris
Coşan, Fulya
Oku, Başar
Özbek, Uğur
Title Cloning of chimerical translocations as positive control for molecular genetic diagnosis of leukemia
Journal name Turkish Journal of Hematology   Check publisher's open access policy
ISSN 1308-5263
Publication date 2008-01-01
Sub-type Article (original research)
Open Access Status File (Publisher version)
Volume 25
Issue 1
Start page 20
End page 23
Total pages 4
Place of publication Ankara, Turkey
Publisher Turk Hematoloji Dernegi
Language eng
Subject 1102 Cardiovascular Medicine and Haematology
Abstract The diagnosis of leukemia-specific mRNAs by polymerase chain reaction (PCR) and reverse transcription-PCR (RT-PCR) require well-known positive standard controls. In general, the positive controls are obtained from cell lines and leukemia patients who have been diagnosed at the molecular level by RT-PCR. These are expensive and restricted sources for standard positive controls. Thus, there is a need for less expensive and reproducible standard positive controls in this area. We have cloned the t (9: 22) p190, t (9: 22) p210, t (4: 11), t (1: 19), t (15: 17), t (12; 21) breakpoint junctions of fusion genes into the plasmids. Cloned fusion genes are suitable for testing PCR experiments of the molecular genetic diagnosis of leukemia samples. We cloned and optimized fusion gene junctions as a standard positive control to check PCR efficiency and as a standard positive marker for diagnosis.
Keyword Fusion genes
Cloning
Reverse transcription-PCR
Q-Index Code C1
Q-Index Status Provisional Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Institute for Molecular Bioscience - Publications
 
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Created: Thu, 25 Mar 2010, 00:29:08 EST by Tara Johnson on behalf of Institute for Molecular Bioscience