[Instability of cryptic plasmids in strain Sinorhizobium meliloti P108 in the course of symbiosis with Alfalfa Medicago sativa]

Roumiantseva, M. L., Andronov, E. E., Sagulenko, V. V., Onishchuk, O. P., Provorov, N. A. and Simarov, B. V. (2004) [Instability of cryptic plasmids in strain Sinorhizobium meliloti P108 in the course of symbiosis with Alfalfa Medicago sativa]. Генетика [Genetika], 40 4: 454-461. doi:10.1023/B:RUGE.0000024971.91460.55


Author Roumiantseva, M. L.
Andronov, E. E.
Sagulenko, V. V.
Onishchuk, O. P.
Provorov, N. A.
Simarov, B. V.
Title [Instability of cryptic plasmids in strain Sinorhizobium meliloti P108 in the course of symbiosis with Alfalfa Medicago sativa]
Formatted title
[Instability of cryptic plasmids in strain Sinorhizobium meliloti P108 in the course of symbiosis with Alfalfa Medicago sativa]
Journal name Генетика [Genetika]   Check publisher's open access policy
ISSN 0016-6758
Publication date 2004-04-01
Sub-type Article (original research)
DOI 10.1023/B:RUGE.0000024971.91460.55
Open Access Status
Volume 40
Issue 4
Start page 454
End page 461
Total pages 8
Place of publication Moskva, Russian Federation
Publisher MAIK Nauka/Interperiodica
Language rus
eng
Subject 0604 Genetics
Formatted abstract
Instability of cryptic plasmids in Sinorhizobium meliloti laboratory strains SXM1, DM7-R, and P108 as well as in their clones isolated from nodules of alfalfa grown during a long-term microvegetation experiment (120 days) was studied. The isolated clones of strains SXM1 and DM7-R manifested stable inheritance of plasmids, whereas 12.7–14.0% of clones with changed plasmid profile were detected in a population of clones from strain P108. These segregants were designated as P108c. Segregants P108c exhibited significantly decreased symbiotic effectiveness, nitrogenase activity, and the competitiveness with respect to alfalfa, compared to the original strain P108. It was established that a 80-kb deletion occurred in a larger of two cryptic plasmids (240 and 230 kb) of segregants P108c. It was concluded that genetic rearrangements are possible in rhizobial clones that did not undergo structural transformation and retained viability in the nodule during the natural vegetation period of alfalfa.
© 2004 MAIK Nauka Interperiodica”
Keyword Medicago sativa
Sinorhizobium meliloti
Nitrogenase
Alfalfa
Rhizobium-leguminosarum
R-meliloti
Agrobacterium-tumefaciens
Sym plasmid
Nodulation
Trifolii
Q-Index Code C1
Additional Notes Translated English version published in Russian Journal of Genetics 40(4) pp.356-362. 1022-7954 (print); 1608-3369 (electronic)

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Chemistry and Molecular Biosciences
 
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