Protonic gating of excited-state twisting and charge localization in GFP chromophores: A mechanistic hypothesis for reversible photoswitching

Olsen, Seth, Lamothe, Kristina and Martinez, Todd J. (2010) Protonic gating of excited-state twisting and charge localization in GFP chromophores: A mechanistic hypothesis for reversible photoswitching. Journal of the American Chemical Society, 132 4: 1192-1193. doi:10.1021/ja907447k


Author Olsen, Seth
Lamothe, Kristina
Martinez, Todd J.
Title Protonic gating of excited-state twisting and charge localization in GFP chromophores: A mechanistic hypothesis for reversible photoswitching
Journal name Journal of the American Chemical Society   Check publisher's open access policy
ISSN 0002-7863
1943-2984
1520-5126
Publication date 2010-02-03
Year available 2010
Sub-type Article (original research)
DOI 10.1021/ja907447k
Volume 132
Issue 4
Start page 1192
End page 1193
Total pages 2
Place of publication Washington, DC, United States
Publisher American Chemical Society
Language eng
Formatted abstract
Reversible photoswitching fluorescent proteins can be photoswitched between fluorescent and nonfluorescent states by different irradiation regimes. Accumulating spectroscopic and crystallographic evidence suggest a correlated change in protonation state and methine bridge isomerism of the chromophore. The anion can decay by photoisomerization of either of the methine bonds, but only one channel can act as a switch. Using ab initio multiple spawning dynamics simulations, we show that protonation is sufficient to change the photoisomerization channel in the chromophore. We propose that this behavior can underlie a switch given certain other conditions. We also propose a basis for coupling between excited-state basicity changes and selection of the photoisomerization channel based on the polarity of twisted charge-transfer states for neutral and anionic forms of the chromophore.
Copyright © 2010 American Chemical Society.
Keyword Green fluorescent protein
Structural basis
Dynamics
Dronpa
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID CHE-09-39169
RGP-0038
DP0877875
M03
Institutional Status UQ
Additional Notes Published under Communications

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Mathematics and Physics
Official 2011 Collection
 
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Created: Sun, 21 Mar 2010, 10:03:48 EST