Rapid loss of glutamine synthetase from astrocytes in response to hypoxia: implications for excitotoxicity

Lee, Aven, Lingwood, Barbara E., Bjorkman, S. Tracey, Miller, Stephanie M., Poronnik, Philip, Barnett, Nigel L., Colditz, Paul and Pow, David V. (2010) Rapid loss of glutamine synthetase from astrocytes in response to hypoxia: implications for excitotoxicity. Journal Of Chemical Neuroanatomy, 39 3: 211-220. doi:10.1016/j.jchemneu.2009.12.002


Author Lee, Aven
Lingwood, Barbara E.
Bjorkman, S. Tracey
Miller, Stephanie M.
Poronnik, Philip
Barnett, Nigel L.
Colditz, Paul
Pow, David V.
Title Rapid loss of glutamine synthetase from astrocytes in response to hypoxia: implications for excitotoxicity
Journal name Journal Of Chemical Neuroanatomy   Check publisher's open access policy
ISSN 0891-0618
1873-6300
Publication date 2010-05-01
Year available 2009
Sub-type Article (original research)
DOI 10.1016/j.jchemneu.2009.12.002
Volume 39
Issue 3
Start page 211
End page 220
Total pages 10
Place of publication Amsterdam, Netherlands
Publisher Elsevier BV
Language eng
Subject C1
060199 Biochemistry and Cell Biology not elsewhere classified
Abstract We have examined brains of neonatal pigs that were rendered hypoxic. Glutamine synthetase (GS), a key enzyme in the detoxification of glutamate and ammonia, was rapidly lost from astrocytes in regions susceptible to damage, including the CA1 of hippocampus and various cortical regions. Conversely, resilient areas such as the dentate gyrus exhibited little or no loss of GS. Onset of loss was rapid, patches of loss being evident by 1h post-insult, and loss was extensive by 24h and did not recover by 72 h. Examination of counterstained sections revealed that GS losses preceded any overt neuronal damage. Loss of GS from astrocytes would plausibly lead to a rise in intracellular glutamate, and could explain why reversal of astrocytic glutamate transport during hypoxia/ischaemia is conceptually possible. © 2009 Elsevier B.V. All rights reserved.
Keyword Alzheimer's Astrocyte
Ammonia
Brain
Glutamate
Ischaemia
Stroke
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online 23 December 2009.

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
2010 Higher Education Research Data Collection
School of Medicine Publications
 
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Created: Thu, 18 Mar 2010, 19:16:18 EST by Ian Sawyer on behalf of UQ Centre for Clinical Research