HBsAg-vectored vaccines simultaneously deliver CTL responses to protective epitopes from multiple viral pathogens

Chen, Dekun, Edgtton, Kristy, Gould, Allan, Guo, Huayang, Mather, Michael, Haigh, Oscar, Cochrane, Melanie, Kattenbelt, Jacqueline, Thomson, Scott and Tindle, Robert (2010) HBsAg-vectored vaccines simultaneously deliver CTL responses to protective epitopes from multiple viral pathogens. Virology, 398 1: 68-78. doi:10.1016/j.virol.2009.11.042

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Author Chen, Dekun
Edgtton, Kristy
Gould, Allan
Guo, Huayang
Mather, Michael
Haigh, Oscar
Cochrane, Melanie
Kattenbelt, Jacqueline
Thomson, Scott
Tindle, Robert
Title HBsAg-vectored vaccines simultaneously deliver CTL responses to protective epitopes from multiple viral pathogens
Journal name Virology   Check publisher's open access policy
ISSN 0042-6822
Publication date 2010-03-01
Year available 2009
Sub-type Article (original research)
DOI 10.1016/j.virol.2009.11.042
Open Access Status Not Open Access
Volume 398
Issue 1
Start page 68
End page 78
Total pages 11
Editor Robert A. Lamb
Place of publication San Diego, CA, U.S.A.
Publisher Elsevier
Language eng
Subject C1
060502 Infectious Agents
060506 Virology
920115 Respiratory System and Diseases (incl. Asthma)
970106 Expanding Knowledge in the Biological Sciences
920108 Immune System and Allergy
920109 Infectious Diseases
Formatted abstract
We have previously demonstrated that the potent immunogenicity of hepatitis B surface antigen (HBsAg) may be exploited to deliver foreign antigens for cytotoxic T-lymphocyte (CTL) induction. Here we demonstrate that a single low-dose immunization with rHBsAg DNA is sufficient to prime for CTL responses against encoded foreign epitope and that the responses may be recalled many months after immunization. We show that simultaneous disease-protective CTL responses restricted through a diversity of MHC class I haplotypes are elicited by recombinant (r) HBsAg DNA containing multiple viral epitopes appended as a C′-terminal polyepitope or encoded individually within the HBsAg polypeptide. CTL responses delivered by rHBsAg DNA were elicited in the presence of HBsAg-directed antibody. These studies vindicate the use of HBsAg as a powerful vector to deliver CTL responses to foreign antigen and have implications for a multidisease vaccine applicable to an MHC-polymorphic population.
© 2009 Elsevier Inc. All rights reserved.
Keyword Recombinant DNA vaccine
Hepatitis B surface antigen
Cytotoxic T-lymphocyte
Vaccine vector
B Surface-antigen
Respiratory Syncytial Virus
DNA-mediated immunization
Human-papillomavirus Type-16
Cytotoxic T-lymphocytes
Polyepitope vaccines
Envelope protein
Transgenic mice
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online 14 December 2009.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Chemistry and Molecular Biosciences
Clinical Medical Virology Centre Publications
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Citation counts: TR Web of Science Citation Count  Cited 1 times in Thomson Reuters Web of Science Article | Citations
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Created: Sun, 07 Mar 2010, 10:00:21 EST