Cloning and expression of candidate sexual development genes in the cane toad (Bufo marinus)

Abramyan, John, Feng, Chun-Wei and Koopman, Peter (2009) Cloning and expression of candidate sexual development genes in the cane toad (Bufo marinus). Developmental Dynamics, 238 9: 2430-2441. doi:10.1002/dvdy.22055


Author Abramyan, John
Feng, Chun-Wei
Koopman, Peter
Title Cloning and expression of candidate sexual development genes in the cane toad (Bufo marinus)
Journal name Developmental Dynamics   Check publisher's open access policy
ISSN 1058-8388
Publication date 2009-09-01
Year available 2009
Sub-type Article (original research)
DOI 10.1002/dvdy.22055
Open Access Status Not yet assessed
Volume 238
Issue 9
Start page 2430
End page 2441
Total pages 12
Editor Gary C. Schoenwolf
Place of publication Hoboken, NJ, U.S.A.
Publisher John Wiley & Sons
Language eng
Subject C1
970106 Expanding Knowledge in the Biological Sciences
060403 Developmental Genetics (incl. Sex Determination)
Abstract The development of the reproductive system in bufonids (true toads) is unique in several respects: sexual differentiation occurs later than in other anurans, and toads develop a Bidder's organ, a rudimentary ovary that can be manipulated in males to produce mature oocytes. To illuminate the genesis of this unusual reproductive system, we isolated from the cane toad (Bufo marinus) the orthologues of several known vertebrate sex-determining genes, determined their primary structure, and studied their expression by reverse transcriptase-polymerase chain reaction and in situ hybridization of tissue sections. We report here that cane toad Sox9, Dmrt1, and p450aromatase (Cyp19a1) are highly homologous to their counterparts in other vertebrates. They show profiles of expression that generally follow patterns observed in other taxa, but with some novel features. Our data suggest that these genes likely play key roles in sex determination and early gonad development in bufonids. Developmental Dynamics 238:2430-2441, 2009. (C) 2009 Wiley-Liss, Inc.
Formatted abstract
The development of the reproductive system in bufonids (true toads) is unique in several respects: sexual differentiation occurs later than in other anurans, and toads develop a Bidder's organ, a rudimentary ovary that can be manipulated in males to produce mature oocytes. To illuminate the genesis of this unusual reproductive system, we isolated from the cane toad (Bufo marinus) the orthologues of several known vertebrate sex-determining genes, determined their primary structure, and studied their expression by reverse transcriptase-polymerase chain reaction and in situ hybridization of tissue sections. We report here that cane toad Sox9, Dmrt1, and p450aromatase (Cyp19a1) are highly homologous to their counterparts in other vertebrates. They show profiles of expression that generally follow patterns observed in other taxa, but with some novel features. Our data suggest that these genes likely play key roles in sex determination and early gonad development in bufonids. Developmental Dynamics 238:2430-2441, 2009. © 2009 Wiley-Liss, Inc.
Keyword cane toad
sex determination
Sox9
Dmrt1
aromatase
gonad
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes First published online August 3 2009

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
Institute for Molecular Bioscience - Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 14 times in Thomson Reuters Web of Science Article | Citations
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Created: Thu, 25 Feb 2010, 01:03:17 EST by Susan Allen on behalf of Institute for Molecular Bioscience