Electroporation and dna-dependent cell-death in murine macrophages

Stacey, KJ, Ross, IL and Hume, DA (1993) Electroporation and dna-dependent cell-death in murine macrophages. Immunology and Cell Biology, 71 2: 75-85. doi:10.1038/icb.1993.8


Author Stacey, KJ
Ross, IL
Hume, DA
Title Electroporation and dna-dependent cell-death in murine macrophages
Journal name Immunology and Cell Biology   Check publisher's open access policy
ISSN 0818-9641
1440-1711
Publication date 1993-04-01
Sub-type Article (original research)
DOI 10.1038/icb.1993.8
Volume 71
Issue 2
Start page 75
End page 85
Total pages 11
Place of publication Basingstoke, U.K.
Publisher Nature Publishing Group
Language eng
Subject 060406 Genetic Immunology
060103 Cell Development, Proliferation and Death
Abstract The difficulty of transfecting primary macrophages and macrophage ceil lines has meant that relatively few studies on regulation of gene expression have been performed in these cells. This study has optimized an electroporation procedure for the macrophage cell line RAW 264, but shows that introduction of DNA into the cytoplasm of primary macrophages by electroporation is toxic to the cells. It is proposed that this cell death may have a physiological role in defence against certain viral infections which result in accumulation of cytoplasmic DNA. RAW 264 cells were efficiently transfected by electroporation, but electroporated bone marrow derived macrophages (BMM) showed large scale cell death over a period of 12 h. Electroporation without DNA was not toxic and DNase treatment of samples before transfection prevented cell death. The toxicity of DNA was concentration-dependent and sequence independent. Synthetic, genomic and plasmid DNA all caused cell death. This sensitivity to DNA seems to be distinct from the antiviral state induced by double-stranded RNA and may be part of an uncharacterized viral defence system.
Keyword cell death
electroporation
macrophage
transfection
DNA toxicity
double-stranded RNA
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Molecular Bioscience - Publications
 
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Created: Fri, 19 Feb 2010, 00:25:07 EST by Laura McTaggart on behalf of Institute for Molecular Bioscience