Megakaryocyte-erythroid lineage promiscuity in EKLF null mouse blood

Tallack, M. R. and Perkins, A. C. (2009) Megakaryocyte-erythroid lineage promiscuity in EKLF null mouse blood. Haematologica - The Haematology Journal, 95 1: 144-147. doi:10.3324/haematol.2009.010017


Author Tallack, M. R.
Perkins, A. C.
Title Megakaryocyte-erythroid lineage promiscuity in EKLF null mouse blood
Journal name Haematologica - The Haematology Journal   Check publisher's open access policy
ISSN 1592-8721
0390-6078
Publication date 2009-10-01
Year available 2009
Sub-type Article (original research)
DOI 10.3324/haematol.2009.010017
Open Access Status DOI
Volume 95
Issue 1
Start page 144
End page 147
Total pages 4
Editor Mario Cazzola
Place of publication Pavia, Italy
Publisher Ferrata Storti Foundation
Language eng
Subject C1
920101 Blood Disorders
060103 Cell Development, Proliferation and Death
Abstract Commitment towards megakaryocyte versus erythroid blood cell lineages occurs in the megakaryocyte-erythroid progenitor, where mutually exclusive expression of either EKLF (Klf1) or Fli1 defines alternative outcomes. Here we show there is a marked increase in the number of circulating platelets in mice lacking the erythroid transcription factor EKLF. In addition, committed erythroid cells retain key signatures of megakaryocytes both on the cell surface and at the mRNA level. We also show that the effect of EKLF on megakaryocyte-erythroid progenitor lineage decision and commitment is cell autonomous in bone marrow reconstitution assays where stem cells lacking EKLF favor the megakaryocyte differentiation pathway. We conclude the megakaryocyte program is aberrantly activated in EKLF null erythroid cells.
Keyword megakaryocyte
EKLF
megakaryocyte differentiation
TRANSCRIPTION FACTORS
MICE
HEMATOPOIESIS
MEGAKARYOPOIESIS
COMMITMENT
PROTEINS
FAMILY
CELLS
FLI-1
GENE
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID 519 718/ACP
DP0770471/ACP
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
Institute for Molecular Bioscience - Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 18 times in Thomson Reuters Web of Science Article | Citations
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Created: Sun, 07 Feb 2010, 10:06:20 EST