Thermal stability of thaumatin-like protein, chitinase, and invertse isolated from sauvignon blanc and semillon juice and their role in haze formation in wine

Falconer, Robert J., Marangon, Matteo, van Sluyter, Steven C., Neilson, Karlie A., Chan, Cherrine and Waters, Elizabeth J. (2010) Thermal stability of thaumatin-like protein, chitinase, and invertse isolated from sauvignon blanc and semillon juice and their role in haze formation in wine. Journal of Agricultural and Food Chemistry, 58 2: 975-980. doi:10.1021/jf902843b


Author Falconer, Robert J.
Marangon, Matteo
van Sluyter, Steven C.
Neilson, Karlie A.
Chan, Cherrine
Waters, Elizabeth J.
Title Thermal stability of thaumatin-like protein, chitinase, and invertse isolated from sauvignon blanc and semillon juice and their role in haze formation in wine
Journal name Journal of Agricultural and Food Chemistry   Check publisher's open access policy
ISSN 0021-8561
1520-5118
Publication date 2010-01-01
Year available 2009
Sub-type Article (original research)
DOI 10.1021/jf902843b
Volume 58
Issue 2
Start page 975
End page 980
Total pages 6
Place of publication Washington, DC, United States
Publisher American Chemical Society
Language eng
Subject C1
Formatted abstract
A thermal unfolding study of thaumatin-like protein, chitinase, and invertase isolated from Vitis vinifera Sauvignon blanc and Semillon juice was undertaken. Differential scanning calorimetry demonstrated that chitinase was a major player in heat-induced haze in unfined wines as it had a low melt temperature, and aggregation was observed. The kinetics of chitinase F1 (Sauvignon blanc) unfolding was studied using circular dichroism spectrometry. Chitinase unfolding conforms to Arrhenius behavior having an activation energy of 320 kJ/mol. This enabled a predictive model for protein stability to be generated, predicting a half-life of 9 years at 15 °C, 4.7 days at 30 °C, and 17 min at 45 °C. Circular dichroism studies indicate that chitinase unfolding follows three steps: an initial irreversible step from the native to an unfolded conformation, a reversible step between a collapsed and an unfolded non-native conformation, followed by irreversible aggregation associated with visible haze formation
Keyword Grape
Denaturation
Aggregation
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
Australian Institute for Bioengineering and Nanotechnology Publications
 
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Created: Sun, 31 Jan 2010, 10:03:14 EST