Experimental vaccination of sheep and cattle against tick infestation using recombinant 5'-nucleotidase

Hope, M., Jiang, X., Gough, J., Cadogan, L., Josh, P., Jonsson, N. and Willadsen, P. (2010) Experimental vaccination of sheep and cattle against tick infestation using recombinant 5'-nucleotidase. Parasite Immunology, 32 2: 135-142. doi:10.1111/j.1365-3024.2009.01168.x


Author Hope, M.
Jiang, X.
Gough, J.
Cadogan, L.
Josh, P.
Jonsson, N.
Willadsen, P.
Title Experimental vaccination of sheep and cattle against tick infestation using recombinant 5'-nucleotidase
Journal name Parasite Immunology   Check publisher's open access policy
ISSN 0141-9838
1365-3024
Publication date 2010-02-01
Sub-type Article (original research)
DOI 10.1111/j.1365-3024.2009.01168.x
Volume 32
Issue 2
Start page 135
End page 142
Total pages 8
Place of publication Oxford, United Kingdom
Publisher Wiley-Blackwell Publishing
Language eng
Formatted abstract
Limited prior evidence suggests that 5′-nucleotidase, an ectoenzyme principally located in the Malpighian tubules of the tick Rhipicephalus (Boophilus) microplus, could be an effective antigen in an anti-tick vaccine. To assess this, recombinant 5′-nucleotidase was expressed in Escherichia coli and used in vaccination trials with both sheep and cattle. Vaccinated sheep were challenged with freshly moulted adult ticks. Those with high titres of anti-nucleotidase antibodies showed significant protection against tick infestation, although protection was less than that found with the previously characterized antigen, Bm86. Cattle were vaccinated, in separate groups, with 5′-nucleotidase, Bm86 and both antigens combined. Cattle, as the natural host, were challenged with larval ticks. Although Bm86 showed typical efficacy, no significant protection was seen in cattle vaccinated with 5′-nucleotidase. Cattle receiving a dual antigen formulation were no better protected than those receiving Bm86 alone. One possible reason for the difference between host species, namely antibody titre, was examined and shown to be an unlikely explanation. This demonstrates a limitation of using a model host like sheep in vaccine studies.
© 2010 Blackwell Publishing Ltd.
Keyword Antigen
Boophilus microplus
Nucleotidase
Rhipicephalus (Boophilus) microplus
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Veterinary Science Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 8 times in Thomson Reuters Web of Science Article | Citations
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Created: Sun, 31 Jan 2010, 10:01:58 EST