Constitutive regulation of the Insl3 gene in rat Leydig cells

Sadeghian, Helen, Anand-Ivell, Ravinder, Balvers, Marga, Relan, Vandana and Ivell, Richard (2005) Constitutive regulation of the Insl3 gene in rat Leydig cells. Molecular and Cellular Endocrinology, 241 1-2: 10-20. doi:10.1016/j.mce.2005.03.017

Author Sadeghian, Helen
Anand-Ivell, Ravinder
Balvers, Marga
Relan, Vandana
Ivell, Richard
Title Constitutive regulation of the Insl3 gene in rat Leydig cells
Formatted title
Constitutive regulation of the Insl3 gene in rat Leydig cells
Journal name Molecular and Cellular Endocrinology   Check publisher's open access policy
ISSN 0303-7207
Publication date 2005-09-28
Sub-type Article (original research)
DOI 10.1016/j.mce.2005.03.017
Volume 241
Issue 1-2
Start page 10
End page 20
Total pages 11
Place of publication Amsterdam, Netherlands
Publisher Elsevier Ireland
Language eng
Subject 0601 Biochemistry and Cell Biology
1103 Clinical Sciences
Formatted abstract
Insulin-like factor 3 (Insl3) is a major new product of the Leydig cells in all mammalian species so far examined. The rat Insl3 gene is encoded by two exons in close juxtaposition to the Jak3 gene. Using RT-PCR analysis we now show that in the rat testis it is expressed as both major and minor splice variants, the former encoding the normal protein, the latter a truncated peptide comprising a C-terminally extended B-domain. Both transcripts are produced in constant relative amounts uniquely in the Leydig cells of the postnatal testis and in no other testicular cell type. Rat Insl3 protein is also expressed only in Leydig cells after postnatal day 30. Although specific mRNA is present at earlier times, corresponding protein is not detected. Semi-quantitative RT-PCR analysis of Insl3 transcripts in the mouse MA-10 tumour Leydig cell-line under a wide range of stimulation regimes shows that in an acute context, the Insl3 gene is expressed absolutely constitutively. This is confirmed by transfection and electrophoretic mobility shift (EMSA) analysis of the rat Insl3 gene promoter, wherein the importance of three putative SF-1 responsive elements is underscored, although these appear to differ in their relative importance from their counterparts in the mouse Insl3 gene.
© 2005 Elsevier Ireland Ltd. All rights reserved.
Keyword Leydig cells
Steroidogenic factor 1
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Medicine Publications
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Created: Thu, 21 Jan 2010, 20:27:53 EST by Jon Swabey on behalf of Faculty Of Health Sciences