Adeno-associated viral vector-mediated gene transfer of brain-derived neurotrophic factor reverses atrophy of rubrospinal neurons following both acute and chronic spinal cord injury

Ruitenberg, Mark J., Blits, Bas, Dijkhuizen, Paul A., te Beek, Eric T., Bakker, Arne, van Heerikhuize, Joop J., Pool, Chris W., Hermens, Wim T. J., Boer, Gerard J. and Verhaagen, Joost (2004) Adeno-associated viral vector-mediated gene transfer of brain-derived neurotrophic factor reverses atrophy of rubrospinal neurons following both acute and chronic spinal cord injury. Neurobiology of Disease, 15 2: 394-406. doi:10.1016/j.nbd.2003.11.018


Author Ruitenberg, Mark J.
Blits, Bas
Dijkhuizen, Paul A.
te Beek, Eric T.
Bakker, Arne
van Heerikhuize, Joop J.
Pool, Chris W.
Hermens, Wim T. J.
Boer, Gerard J.
Verhaagen, Joost
Title Adeno-associated viral vector-mediated gene transfer of brain-derived neurotrophic factor reverses atrophy of rubrospinal neurons following both acute and chronic spinal cord injury
Journal name Neurobiology of Disease   Check publisher's open access policy
ISSN 0969-9961
1095-953X
Publication date 2004-03-01
Sub-type Article (original research)
DOI 10.1016/j.nbd.2003.11.018
Volume 15
Issue 2
Start page 394
End page 406
Total pages 13
Editor W. C. Mobley
S. Gilman
Place of publication Orlando, Florida, U.S.A.
Publisher Academic Press
Language eng
Subject 1103 Clinical Sciences
Abstract Rubrospinal neurons (RSNs) undergo marked atrophy after cervical axotomy. This progressive atrophy may impair the regenerative capacity of RSNs in response to repair strategies that are targeted to promote rubrospinal tract regeneration. Here, we investigated whether we could achieve long-term rescue of RSNs from lesion-induced atrophy by adeno-associated viral (AAV) vector-mediated gene transfer of brain-derived neurotrophic factor (BDNF). We show for the first time that AAV vectors can be used for the persistent transduction of highly atrophic neurons in the red nucleus (RN) for up to 18 months after injury. Furthermore, BDNF gene transfer into the RN following spinal axotomy resulted in counteraction of atrophy in both the acute and chronic stage after injury. These novel findings demonstrate that a gene therapeutic approach can be used to reverse atrophy of lesioned CNS neurons for an extended period of time. © 2004 Elsevier Inc. All rights reserved.
Keyword Adeno-associated viral vector
Atrophy
Brain-derived neurotrophic factor
Gene therapy
Regeneration
Rubrospinal tract
Spinal cord injury
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Biomedical Sciences Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 53 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 67 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Wed, 20 Jan 2010, 21:14:48 EST by Kelly Whitehorne on behalf of Faculty of Science