Expression of the insulin-like peptide 3 (INSL3) hormone-receptor (LGR8) system in the testis

Anand-Ivell, Ravinder J. K., Relan, Vandana, Balvers, Marga, Coiffec-Dorval, Isabelle, Fritsch, Martin, Bathgate, Ross A. D. and Ivell, Richard (2006) Expression of the insulin-like peptide 3 (INSL3) hormone-receptor (LGR8) system in the testis. Biology of Reproduction, 74 5: 945-953. doi:10.1095/biolreprod.105.048165

Author Anand-Ivell, Ravinder J. K.
Relan, Vandana
Balvers, Marga
Coiffec-Dorval, Isabelle
Fritsch, Martin
Bathgate, Ross A. D.
Ivell, Richard
Title Expression of the insulin-like peptide 3 (INSL3) hormone-receptor (LGR8) system in the testis
Journal name Biology of Reproduction   Check publisher's open access policy
ISSN 0006-3363
Publication date 2006-05-01
Sub-type Article (original research)
DOI 10.1095/biolreprod.105.048165
Volume 74
Issue 5
Start page 945
End page 953
Total pages 9
Place of publication Madison, WI, U.S.A.
Publisher Society for the Study of Reproduction
Language eng
Subject 1114 Paediatrics and Reproductive Medicine
0707 Veterinary Sciences
Formatted abstract
The new peptide hormone insulin-like peptide 3 (INSL3) is a member of the insulin-relaxin family, yet, unlike insulin, it signals through a new G-protein coupled receptor, LGR8, distantly related to the receptors for LH and FSH. INSL3 is produced in large amounts by the Leydig cells of the testis in both fetal and adult mammals. Using a combination of mRNA analysis by RT-PCR, immunohistochemistry, ligand-binding, and/or bioactivity assays, the distribution of LGR8 expression was assessed in testicular tissues and cells and in the epididymis. There was consistent agreement that LGR8 was expressed in meiotic and particularly postmeiotic germ cells and in Leydig cells, though not in Sertoli or peritubular cells. Leydig cells appear to express only a low level of the LGR8 gene product; other transcripts may be present, representing nonfunctional products. Messenger RNA analysis suggested that LGR8 transcripts in germ cells represented mostly full-length forms. LGR8 mRNA was also expressed in the epididymis, though no function can yet be ascribed to this expression. Therefore, the INSL3/LGR8 system represents a further paracrine hormone-receptor system in the testis, which conveys information about Leydig cell status to germ cells, and possibly as part of an autocrine feedback loop.
Keyword Peptide 3 (INSL3)
LGR8 expression
Leydig cells
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Medicine Publications
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Created: Fri, 15 Jan 2010, 22:30:51 EST by June Temby on behalf of Faculty Of Health Sciences