Selective permeabilization of the host cell membrane of Plasmodium falciparum-infected red blood cells with streptolysin 0 and equinatoxin II

Jackson, Katherine E., Spielmann, Tobias, Hanssen, Eric, Adisa, Akinola, Separovic, Frances, Dixon, Matthew W. A., Trenholme, Katharine R., Hawthorne, Paula L., Gardiner, Don L., Gilberger, Tim and Tilley, Leann (2007) Selective permeabilization of the host cell membrane of Plasmodium falciparum-infected red blood cells with streptolysin 0 and equinatoxin II. Biochemical journal, 403 1: 167-175. doi:10.1042/BJ20061725


Author Jackson, Katherine E.
Spielmann, Tobias
Hanssen, Eric
Adisa, Akinola
Separovic, Frances
Dixon, Matthew W. A.
Trenholme, Katharine R.
Hawthorne, Paula L.
Gardiner, Don L.
Gilberger, Tim
Tilley, Leann
Title Selective permeabilization of the host cell membrane of Plasmodium falciparum-infected red blood cells with streptolysin 0 and equinatoxin II
Formatted title
Selective permeabilization of the host cell membrane of Plasmodium falciparum-infected red blood cells with streptolysin 0 and equinatoxin II
Journal name Biochemical journal   Check publisher's open access policy
ISSN 0006-2936
0264-6021
Publication date 2007-04-01
Sub-type Article (original research)
DOI 10.1042/BJ20061725
Volume 403
Issue 1
Start page 167
End page 175
Total pages 9
Place of publication London, U.K.
Publisher Portland Press
Language eng
Subject 0601 Biochemistry and Cell Biology
Formatted abstract
Plasmodium falciparum develops within the mature RBCs (red blood cells) of its human host in a PV (parasitophorous vacuole) that separates the host cell cytoplasm from the parasite surface. The pore-forming toxin, SLO (streptolysin O), binds to cholesterol-containing membranes and can be used to selectively permeabilize the host cell membrane while leaving the PV membrane intact. We found that in mixtures of infected and uninfected RBCs, SLO preferentially lyses uninfected RBCs rather than infected RBCs, presumably because of differences in cholesterol content of the limiting membrane. This provides a means of generating pure preparations of viable ring stage infected RBCs. As an alternative permeabilizing agent we have characterized EqtII (equinatoxin II), a eukaryotic pore-forming toxin that binds preferentially to sphingomyelin-containing membranes. EqtII lyses the limiting membrane of infected and uninfected RBCs with similar efficiency but does not disrupt the PV membrane. It generates pores of up to 100 nm, which allow entry of antibodies for immunofluorescence and immunogold labelling. The present study provides novel tools for the analysis of this important human pathogen and highlights differences between Plasmodium-infected and uninfected RBCs. The Biochemical Society, London © 2007.
Keyword Cholesterol
Equinatoxin II
Malaria
Plasmodium falciparum
Pore-forming toxin
Streptolysin O
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Australian Equine Genetics Research Centre Publications
 
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Created: Tue, 12 Jan 2010, 21:12:54 EST by Tara Johnson on behalf of Aust Equine Genetics Research Centre