Rapid determination of sulfonamides in milk using micellar electrokinetic chromatography with fluorescence detection

Lamba, Sushma, Sanghi, Sunil Kumar, Asthana, Amit and Shelke, Manjusha (2005) Rapid determination of sulfonamides in milk using micellar electrokinetic chromatography with fluorescence detection. Analytica Chimica Acta, 552 1-2: 110-115. doi:10.1016/j.aca.2005.05.084


Author Lamba, Sushma
Sanghi, Sunil Kumar
Asthana, Amit
Shelke, Manjusha
Title Rapid determination of sulfonamides in milk using micellar electrokinetic chromatography with fluorescence detection
Journal name Analytica Chimica Acta   Check publisher's open access policy
ISSN 0003-2670
1873-4324
Publication date 2005-11-03
Sub-type Article (original research)
DOI 10.1016/j.aca.2005.05.084
Open Access Status Not Open Access
Volume 552
Issue 1-2
Start page 110
End page 115
Total pages 6
Place of publication Amsterdam, Netherlands
Publisher Elsevier BV
Language eng
Subject 0301 Analytical Chemistry
Abstract A new method was developed for the determination of sulfonamides in milk by using micellar electrokinetic chromatography coupled with fluorescence detection. Separation of fluorescamine-derivatized sulfonamides was accomplished by using a buffer 13.32 mM disodium hydrogen phosphate, 6.67 mM potassium dihydrogen phosphate and 40 mM sodium dodecyl sulphate at pH 7.5 in addition to positive power supply at 21 kV at 25 °C. Detection was performed using UG-11 excitation filter and 495 nm emission filters. The proposed capillary electrophoresis method allows the separation of five sulfonamides within 7 min with a limit of detection of 1.59–7.68 nmol/L for all the sulfonamides considered for present study. A simple sample preparation method with fairly good recoveries 85–114% is also presented in current paper. Inter-day and intra-day validation of the separation method shows fairly good results. Robustness of the method has also been studied. © 2005 Elsevier B.V. All rights reserved.
Keyword Sulfonamides
Fluorescamine
Micellar electrokinetic chromatography
Milk
Q-Index Code C1
Additional Notes Available online 3 October 2005

 
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Created: Mon, 11 Jan 2010, 23:55:37 EST by Jon Swabey on behalf of Aust Institute for Bioengineering & Nanotechnology