Z and W sex chromosomes in the cane toad (Bufo marinus)

Abramyan, John, Ezaz, Tariq, Graves, Jennifer A. Marshall and Koopman, Peter (2009) Z and W sex chromosomes in the cane toad (Bufo marinus). Chromosome Research, 17 8: 1015-1024. doi:10.1007/s10577-009-9095-1

Author Abramyan, John
Ezaz, Tariq
Graves, Jennifer A. Marshall
Koopman, Peter
Title Z and W sex chromosomes in the cane toad (Bufo marinus)
Formatted title
Z and W sex chromosomes in the cane toad (Bufo marinus)
Journal name Chromosome Research   Check publisher's open access policy
ISSN 0967-3849
Publication date 2009-12-01
Year available 2009
Sub-type Article (original research)
DOI 10.1007/s10577-009-9095-1
Volume 17
Issue 8
Start page 1015
End page 1024
Total pages 10
Editor Herbert C Macgregor
Place of publication Dordrecht, Netherlands
Publisher Springer Netherlands
Language eng
Subject 970106 Expanding Knowledge in the Biological Sciences
060403 Developmental Genetics (incl. Sex Determination)
0604 Genetics
Formatted abstract
The cane toad (Bufo marinus) is one of the most notorious animal pests encountered in Australia. Members of the genus Bufo historically have been regarded as having genotypic sex determination with male homogamety/female heterogamety. Nevertheless, as with many toads, karyotypic analyses of the cane toad have so far failed to identify heteromorphics sex chromosomes. In this study, we used comparative genomic hybridization, reverse fluorescence staining, C-banding, and morphometric analyses of chromosomes to characterize sex chromosome dimorphism in B. marinus. We found that females consistently had a length dimorphism associated with a nucleolus organizer region (NOR) on one of the chromosome 7 pair. A strong signal over the longer NOR in females, and the absence of a signal in males indicated sex-specific DNA sequences. All females were heterozygous and all males homozygous, indicating a ZZ/ZW sex chromosomal system. Our study confirms the existence of sex chromosomes in this species. The ability to reliably identify genotypic sex of cane toads will be of value in monitoring and control efforts in Australia and abroad.
Keyword Cane toad
Sex chromosomes
Nucleolus organizer region
Chromomycin A3
Bufo marinus
Nucleolar organizer regions
Frog Rana-rugosa
Heterogametic sex
Linked genes
Genus bufo
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Electronic supplementary material The online version of this article (doi:10.1007/s10577-009-9095-1) contains supplementary material, which is available to authorized users.

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
Institute for Molecular Bioscience - Publications
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Citation counts: TR Web of Science Citation Count  Cited 17 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 18 times in Scopus Article | Citations
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Created: Sun, 03 Jan 2010, 10:03:55 EST