Global Survey of Protein Expression during Gonadal Sex Determination in Mice

Ewen, K, Baker, M. A., Wilhelm, D, Aitken, R. J. and Koopman, P. A. (2009) Global Survey of Protein Expression during Gonadal Sex Determination in Mice. Molecular and Cellular Proteomics, 8 12: 2624-2641. doi:10.1074/mcp.M900108-MCP200


Author Ewen, K
Baker, M. A.
Wilhelm, D
Aitken, R. J.
Koopman, P. A.
Title Global Survey of Protein Expression during Gonadal Sex Determination in Mice
Journal name Molecular and Cellular Proteomics   Check publisher's open access policy
ISSN 1535-9476
Publication date 2009-12-01
Year available 2009
Sub-type Article (original research)
DOI 10.1074/mcp.M900108-MCP200
Open Access Status Not yet assessed
Volume 8
Issue 12
Start page 2624
End page 2641
Total pages 18
Editor Alma L Burlingame
Place of publication Bethesda, MD, U. S. A.
Publisher American Society for Biochemistry and Molecular Biology
Language eng
Subject C1
970106 Expanding Knowledge in the Biological Sciences
060403 Developmental Genetics (incl. Sex Determination)
Abstract The development of an embryo as male or female depends on differentiation of the gonads as either testes or ovaries. A number of genes are known to be important for gonadal differentiation, but our understanding of the regulatory networks underpinning sex determination remains fragmentary. To advance our understanding of sexual development beyond the transcriptome level, we performed the first global survey of the mouse gonad proteome at the time of sex determination by using two-dimensional nanoflow LC-MS/MS. The resulting data set contains a total of 1037 gene products (154 non-redundant and 883 redundant proteins) identified from 620 peptides. Functional classification and biological network construction suggested that the identified proteins primarily serve in RNA post-transcriptional modification and trafficking, protein synthesis and folding, and post-translational modification. The data set contains potential novel regulators of gonad development and sex determination not revealed previously by transcriptomics and proteomics studies and more than 60 proteins with potential links to human disorders of sexual development.
Keyword PRIMORDIAL GERM-CELLS
CANDIDATE SPERMATOGENESIS GENE
UBIQUITIN-ACTIVATING ENZYME-E1
EMBRYONIC STEM-CELLS
MOUSE Y-CHROMOSOME
TARGETED DISRUPTION
OVARIAN DEVELOPMENT
TESTIS DEVELOPMENT
PARTIAL TRISOMY
RNA-HELICASE
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
Institute for Molecular Bioscience - Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 8 times in Thomson Reuters Web of Science Article | Citations
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Created: Sun, 27 Dec 2009, 10:01:16 EST