Profiles of gonadal gene expression in the developing bovine embryo

Ross, D.G.F., Bowles, J., Hope, M., Lehnert, S. and Koopman, P. (2009) Profiles of gonadal gene expression in the developing bovine embryo. Sexual Development, 3 5: 273-283. doi:10.1159/000252791


Author Ross, D.G.F.
Bowles, J.
Hope, M.
Lehnert, S.
Koopman, P.
Title Profiles of gonadal gene expression in the developing bovine embryo
Journal name Sexual Development   Check publisher's open access policy
ISSN 1661-5425
Publication date 2009-12-01
Year available 2009
Sub-type Article (original research)
DOI 10.1159/000252791
Open Access Status Not Open Access
Volume 3
Issue 5
Start page 273
End page 283
Total pages 11
Editor G. Scherer
M. Schartl
M. Schmid
Place of publication Basel, Switzerland
Publisher S. Karger AG
Language eng
Subject 0604 Genetics
C1
970106 Expanding Knowledge in the Biological Sciences
060403 Developmental Genetics (incl. Sex Determination)
Abstract Our current understanding of the molecular basis of sex determination and gonadal development in humans is mostly an extrapolation of knowledge gained from studies in the mouse. However, the timing of gene expression in the mouse is unusual among mammals, and it is therefore important that data from other models are also available to help elucidate this pivotal process in human development. Here we describe the sequence of molecular and morphological events marking testis differentiation in bovine embryos. The genital ridges first appeared at CRL 12 (day 32). SRY expression began at CRL 18 (day 37) and peaked at CRL 20 (day 39), leading to a cascade of regulatory, signaling, and steroidogenic gene expression at later stages, detected by quantitative real-time RT-PCR and immunohistochemistry. Testis cords were distinguishable at CRL 27 (day 42). We conclude that the timing of gene expression observed in developing human embryos is much more similar to bovine development than it is to the mouse. Therefore, Bos taurus may represent a useful model in which to study gene expression during sex determination, relevant to human development.
Keyword Bovine
Development
Embryo
Genital ridge
Gonad
Sex determination
SRY
Testis
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 9 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 8 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Sun, 27 Dec 2009, 10:00:33 EST