Rapidly evolving CRISPRs implicated in acquired resistance of microorganisms to viruses

Tyson, Gene W. and Banfield, Jillian F. (2008) Rapidly evolving CRISPRs implicated in acquired resistance of microorganisms to viruses. Environmental Microbiology, 10 1: 200-207. doi:10.1111/j.1462-2920.2007.01444.x


Author Tyson, Gene W.
Banfield, Jillian F.
Title Rapidly evolving CRISPRs implicated in acquired resistance of microorganisms to viruses
Journal name Environmental Microbiology   Check publisher's open access policy
ISSN 1462-2912
1462-2920
Publication date 2008-01-01
Year available 2008
Sub-type Article (original research)
DOI 10.1111/j.1462-2920.2007.01444.x
Open Access Status
Volume 10
Issue 1
Start page 200
End page 207
Total pages 8
Place of publication Oxford, U.K.
Publisher Blackwell Science
Language eng
Subject 03 Chemical Sciences
0304 Medicinal and Biomolecular Chemistry
06 Biological Sciences
0601 Biochemistry and Cell Biology
Abstract Recent experimental evidence has demonstrated that bacteria acquire resistance to viruses by incorporation of short transcribed nucleotide sequences into regions of clustered regularly interspaced short palindromic repeats (CRISPR). We have analysed community genomic data from acidophilic microbial biofilms and discovered that evolution of the CRISPR regions in two distinct Leptospirillum group II bacteria occurs fast enough to promote individuality in otherwise nearly clonal populations. Comparative genomics strongly indicates very recent lateral transfer of the CRISPR locus between these populations, followed by significant loss of spacer sequences and locus expansion by unidirectional heterogeneous addition of new spacer sequences. Diversification of the CRISPR region is inferred to be a population-level response to the rapidly changing selective pressure of phage predation. Results reinforce the importance of phage–host interactions in shaping microbial ecology and evolution over very short time scales.
Keyword CRIPSRs
Evolution
Bacteria
Resistance
genomics
Microorganisms
Viruses
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Chemistry and Molecular Biosciences
 
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Created: Tue, 22 Dec 2009, 20:46:42 EST by Macushla Boyle on behalf of Faculty of Science