Community proteomics of a natural microbial biofilm

Ram, Rachna J., VerBerkmoes, Nathan C., Thelen, Michael P., Tyson, Gene W., Baker, Brett J., Blake, Robert C., Shah, Manesh, Hettich, Robert L. and Banfield, Jillian F. (2005) Community proteomics of a natural microbial biofilm. Science, 308 5730: 1915-1920. doi:10.1126/science.1109070


Author Ram, Rachna J.
VerBerkmoes, Nathan C.
Thelen, Michael P.
Tyson, Gene W.
Baker, Brett J.
Blake, Robert C.
Shah, Manesh
Hettich, Robert L.
Banfield, Jillian F.
Title Community proteomics of a natural microbial biofilm
Journal name Science   Check publisher's open access policy
ISSN 1095-9203
0036-8075
Publication date 2005-06-24
Year available 2005
Sub-type Article (original research)
DOI 10.1126/science.1109070
Open Access Status
Volume 308
Issue 5730
Start page 1915
End page 1920
Total pages 6
Place of publication Washington, D.C. USA
Publisher American Association for the Advancement of Science
Language eng
Subject 06 Biological Sciences
0605 Microbiology
Abstract Using genomic and mass spectrometry–based proteomic methods, we evaluated gene expression, identified key activities, and examined partitioning of metabolic functions in a natural acid mine drainage (AMD) microbial biofilm community. We detected 2033 proteins from the five most abundant species in the biofilm, including 48% of the predicted proteins from the dominant biofilm organism, Leptospirillum group II. Proteins involved in protein refolding and response to oxidative stress appeared to be highly expressed, which suggests that damage to biomolecules is a key challenge for survival. We validated and estimated the relative abundance and cellular localization of 357 unique and 215 conserved novel proteins and determined that one abundant novel protein is a cytochrome central to iron oxidation and AMD formation.
Keyword Acid-mine Drainage
Leptospirillum-ferrooxidans
Shotgun Proteomics
Mass-spectrometry
Yeast proteome
Iron
identification
Chromatography
metabolism
Peptide
Q-Index Code C1
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Chemistry and Molecular Biosciences
 
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Created: Fri, 18 Dec 2009, 02:29:40 EST by Maria Campbell on behalf of Faculty of Science