Multiple paternity in Rhipicephalus (Boophilus) microplus confirmed by microsatellite analysis

Cutulle, C., Jonsson, N.N. and Seddon, J.M. (2010) Multiple paternity in Rhipicephalus (Boophilus) microplus confirmed by microsatellite analysis. Experimental And Applied Acarology, 50 1: 51-58. doi:10.1007/s10493-009-9298-3


Author Cutulle, C.
Jonsson, N.N.
Seddon, J.M.
Title Multiple paternity in Rhipicephalus (Boophilus) microplus confirmed by microsatellite analysis
Journal name Experimental And Applied Acarology   Check publisher's open access policy
ISSN 0168-8162
Publication date 2010-01-01
Year available 2009
Sub-type Article (original research)
DOI 10.1007/s10493-009-9298-3
Open Access Status Not yet assessed
Volume 50
Issue 1
Start page 51
End page 58
Total pages 8
Editor Frans Jongejan
J. Bruin
Place of publication Netherlands
Publisher Springer
Language eng
Subject C1
Abstract The aim of this study was to determine if individual ticks among the progeny of a single female Rhipicephalus (Boophilus) microplus tick removed from cattle under natural conditions are the result of mating with one or several males. To this end, simulations were run using an existing dataset of genotypes from 8 microsatellite loci to predict the number of samples required and the best locus. Subsequently, 14-22 progeny from each of 15 engorged female ticks removed from three cows, and the engorged females themselves, were genotyped for the BmM1 locus and the minimum number of potential male parents was determined for each progeny group. Of the 15 progeny groups, 10 must have been sired by more than one male, as indicated by the presence of five unique alleles among the progeny or three unique alleles that could not have been contributed by the female. This finding demonstrates multiple paternity in R. microplus.
Keyword Rhipicephalus (Boophilus) microplus
Cattle tick
Mating systems
Microsatellite
Paternity analysis
CATTLE TICK
IXODIDAE
ACARI
PARTHENOGENESIS
FERTILIZATION
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
School of Veterinary Science Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 5 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 5 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Sun, 13 Dec 2009, 10:06:04 EST