Understanding plasmid effect on hyaluronic acid molecular weight produced by Streptococcus equi subsp. zooepidemicus

Marcellin, Esteban, Chen, Wendy Y. and Nielsen, Lars K. (2010) Understanding plasmid effect on hyaluronic acid molecular weight produced by Streptococcus equi subsp. zooepidemicus. Metabolic Engineering, 12 1: 62-69. doi:10.1016/j.ymben.2009.09.001


Author Marcellin, Esteban
Chen, Wendy Y.
Nielsen, Lars K.
Title Understanding plasmid effect on hyaluronic acid molecular weight produced by Streptococcus equi subsp. zooepidemicus
Formatted title
Understanding plasmid effect on hyaluronic acid molecular weight produced by Streptococcus equi subsp. zooepidemicus
Journal name Metabolic Engineering   Check publisher's open access policy
ISSN 1096-7176
1096-7184
Publication date 2010-01-01
Year available 2009
Sub-type Article (original research)
DOI 10.1016/j.ymben.2009.09.001
Volume 12
Issue 1
Start page 62
End page 69
Total pages 8
Place of publication San Diego, CA, United States
Publisher Academic Press
Language eng
Formatted abstract
Hyaluronic acid is a biopolymer with valuable applications in the pharmaceutical and cosmetic industries. Streptococcus equi subspecies zooepidemicus cells transformed with a nisin-inducible, empty plasmid control displayed higher molecular weight. This increase in molecular weight is independent of the nisin promoter or antibiotic resistance. Using 2D DIGE followed by mass spectrometry, we identified up-regulation of the last step in UDP-N-acetyl-glucosamine biosynthesis (GlmU) and down-regulation of the first step in peptidoglycan biosynthesis (MurA) as possible mechanism for the plasmid effect. Over-expression of GlmU to further increase activity had no effect on UDP-N-acetyl-glucosamine levels or molecular weight, while over-expression of MurA reduced UDP-N-acetyl-glucosamine levels and molecular weight. Global transcriptional analysis revealed that differential regulation of GlmU and MurA activity was not reflected in transcription levels. This results, suggest that regulation is at a translational or post-translational level. Differential expression of two clp proteases may explain this effect as well as the small but significant changes in transcription levels of nearly 300 genes.
© 2009 Elsevier Inc. All rights reserved.
Keyword Hyaluronic acid
Streptococcus zooepidemicus
Plasmid effect
Proteomics
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online 23 September 2009

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
Australian Institute for Bioengineering and Nanotechnology Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 13 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 14 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Sun, 06 Dec 2009, 10:03:49 EST