D-2-receptor-linked signaling pathways regulate the expression of hepatic CYP2E1

Konstandi, Maria, Harkitis, Panagiotis, Kostakis, Dimitris, Marselos, Marios, Johnson, Elizabeth O. and Lang, Matti A. (2008) D-2-receptor-linked signaling pathways regulate the expression of hepatic CYP2E1. Life Sciences, 82 1-2: 1-10. doi:10.1016/j.lfs.2007.09.026

Author Konstandi, Maria
Harkitis, Panagiotis
Kostakis, Dimitris
Marselos, Marios
Johnson, Elizabeth O.
Lang, Matti A.
Title D-2-receptor-linked signaling pathways regulate the expression of hepatic CYP2E1
Journal name Life Sciences   Check publisher's open access policy
ISSN 0024-3205
Publication date 2008-01-02
Sub-type Article (original research)
DOI 10.1016/j.lfs.2007.09.026
Open Access Status Not yet assessed
Volume 82
Issue 1-2
Start page 1
End page 10
Total pages 10
Place of publication New York, USA
Publisher Elsevier
Language eng
Subject 11 Medical and Health Sciences
Abstract This study investigated the role of catecholamine-related signaling pathways in the regulation of hepatic cytochrome P450 (CYP2E1). Central and peripheral catecholamine depletion with reserpine down-regulated CYP2E1. On the other hand, selective peripheral catecholamine depletion with guanethidine increased CYP2E1 apoprotein levels. Enrichment of peripheral catecholamines with adrenaline suppressed p-nitrophenol hydroxylase activity (PNP). PNP activity was also markedly suppressed by l-DOPA. Stimulation of D2-receptors with bromocriptine up-regulated CYP2E1, as assessed by enzyme activity and protein levels, whereas blockade of D2-dopaminergic receptors with sulpiride down-regulated this isozyme. These findings indicate that central and peripheral catecholamines have different effects on CYP2E1. Central catecholamines appear related to the up-regulation, whereas the role of peripheral catecholamines is clearly related to the type and location of adrenoceptors involved. D2-receptor-linked signaling pathways have an up-regulating effect on CYP2E1, while D1-receptor pathways may down-regulate this isozyme. It is worth noting that the widespread environmental pollutant benzo(α)pyrene (B(α)P) altered the modulating effect of catecholaminergic systems on CYP2E1 regulation. In particular, whereas stimulation or blockade of adrenoceptors had no effect on constitutive PNP activity, exposure to B(α)P modified the impact of central and peripheral catecholamines and α2-adrenoceptors on CYP2E1 expression. It appears that under the influence of B(α)P, α2-adrenergic receptor-linked signaling pathways increased CYP2E1 apoprotein levels. Given that a wide range of xenobiotics and clinically used drugs are activated by CYP2E1 to toxic metabolites, including the production of reactive oxygen species (ROS), it is possible that therapies challenging dopaminergic receptor- and/or alpha2-adrenoceptor-linked signaling pathways may alter the expression of CYP2E1, thus affecting the progress and development of several pathologies.
Keyword Dopamine
Polycyclic Aromatic-hydrocarbons
Posttranscriptional Regulation
Cytochromes P450
Q-Index Code C1
Q-Index Status Provisional Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
National Research Centre for Environmental Toxicology Publications
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Citation counts: TR Web of Science Citation Count  Cited 9 times in Thomson Reuters Web of Science Article | Citations
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Created: Tue, 01 Dec 2009, 01:03:50 EST