Selective induction of coumarin 7-hydroxylase by pyrazole in D2 mice

Juvonen, Risto O., Kaipainen, Pekka K. and Lang, Matti A. (1985) Selective induction of coumarin 7-hydroxylase by pyrazole in D2 mice. European Journal of Biochemistry, 152 1: 3-8. doi:10.1111/j.1432-1033.1985.tb09156.x


Author Juvonen, Risto O.
Kaipainen, Pekka K.
Lang, Matti A.
Title Selective induction of coumarin 7-hydroxylase by pyrazole in D2 mice
Formatted title
Selective induction of coumarin 7-hydroxylase by pyrazole in D2 mice
Journal name European Journal of Biochemistry   Check publisher's open access policy
ISSN 0014-2956
1432-1033
Publication date 1985-10-01
Sub-type Article (original research)
DOI 10.1111/j.1432-1033.1985.tb09156.x
Volume 152
Issue 1
Start page 3
End page 8
Total pages 6
Place of publication Oxford, United Kingdom
Publisher Wiley-Blackwell Publishing
Language eng
Formatted abstract
Pyrazole, was given to DBA/2N (D2), C57BL/6N (B6) and AKR/N mice to study its effects on hepatic drug metabolism. A decrease in the total amount of microsomal cytochrome P-450 as well as in the activities of ethylmorphine demethylase and benzo[a]pyrene hydroxylase was found. On the other hand ethoxycoumarin de-ethylase was increased 1.5-2.5-fold (depending on the strain of mouse) and coumarin 7-hydroxylase as much as sevenfold (but only in D2 mice) after pyrazole tretment. This increase was much higher than that caused by phenobarbital, the only well known inducer of coumarin 7-hydroxylase. By reconstituting the mono-oxygenase complex after purification of cytochrome P-450 we found a 40-fold increase in coumarin 7-hydroxylase and eightfold increase in ethoxycoumarin de-ethylase after pyrazole treatment. This was found only in D2 mice. An antibody previously developed against a cytochrome P-450 fraction from the D2 strain with a high coumarin 7-hydroxylase activity inhibited the microsomal coumarin 7-hydroxylase almost 100% after pyrazole pretreatment of the animals. In the case of control or phenobarbital-treated mice the inhibition was somewhat weaker. With the reconstituted mono-oxygenase complex the inhibition of coumarin 7-hydroxylase was almost 100% both for control and pyrazole-treated D2 mice. The data indicate that pyrazole causes an induction of the microsomal monooxygenase complex different from that caused by phenobarbital or 3-methylcholanthrene and selective for coumarin 7-hydroxylation or 7-ethoxycoumarin de-ethylation. This induction was strong in D2, weak in B6 and absent in AKR/N mice.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Journal continues (2005-) The FEBS Journal (1742-4658; 1742-464X)

Document type: Journal Article
Sub-type: Article (original research)
Collection: National Research Centre for Environmental Toxicology Publications
 
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Created: Tue, 01 Dec 2009, 01:02:58 EST