Protective efficacy of the antioxidants vitamin e and trolox against Microcystis aeruginosa and microcystin-LR in Artemia franciscana nauplii

Ruebhart, David R., Wickramasinghe, Wasantha and Cock, Ian E. (2009) Protective efficacy of the antioxidants vitamin e and trolox against Microcystis aeruginosa and microcystin-LR in Artemia franciscana nauplii. Journal of Toxicology and Environmental Health Part A, 72 24: 1567-1575. doi:10.1080/15287390903232459


Author Ruebhart, David R.
Wickramasinghe, Wasantha
Cock, Ian E.
Title Protective efficacy of the antioxidants vitamin e and trolox against Microcystis aeruginosa and microcystin-LR in Artemia franciscana nauplii
Formatted title
Protective efficacy of the antioxidants vitamin e and trolox against Microcystis aeruginosa and microcystin-LR in Artemia franciscana nauplii
Journal name Journal of Toxicology and Environmental Health Part A   Check publisher's open access policy
ISSN 1528-7394
1087-2620
Publication date 2009-01-01
Sub-type Article (original research)
DOI 10.1080/15287390903232459
Volume 72
Issue 24
Start page 1567
End page 1575
Total pages 9
Place of publication Philadelphia, PA, United States
Publisher Taylor & Francis
Language eng
Formatted abstract
This study was undertaken to evaluate the protective efficacy of the antioxidants vitamin E and Trolox (a water-soluble vitamin E derivative) against the toxicity of microcystin-LR (MC-LR), Microcystis aeruginosa aqueous extract (CE), and a reference toxin, menadione sodium bisulfite (MSB), in Artemia franciscana nauplii. This was achieved by using the well-established brine shrimp bioassay. The experiment was conducted in 2 stages, with (1) 12-h mortality time course and (2) LC50 determination for 12- and 24-h exposures. Treatments consisted of MC-LR, CE, and MSB alone and with 4-h pretreatments of either vitamin E or Trolox. Sensitivity of A. franciscana nauplii with 24-h LC50 values of 11 (10.1–12.1) μg/ml for MSB and 9.5 (8.8–10.4) μg/ml for MC-LR were in general agreement with values reported for Artemia sp. Both antioxidant pretreatments resulted in significant reductions in mortality of approximately 50% at 9 h postexposure when challenged by either 40 μg/ml MC-LR or 20 μg/ml MSB. In contrast, the antioxidant pretreatments offered little to no protection from CE, suggesting that other uncharacterized bioactive compounds contributed to overall toxicity. The described bioassay is easily accessible, inexpensive, rapid, and complies with animal ethics guidelines of many countries, and thus provides a potential alternative to the mouse bioassay for the initial screening for chemoprotectants against MC-LR toxicity.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: National Research Centre for Environmental Toxicology Publications
 
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Created: Sun, 22 Nov 2009, 10:02:29 EST