A high sensitivity assay for the inflammatory marker C-Reactive protein employing acoustic biosensing

McBride, Jeffrey D. and Cooper, Matthew A. (2008) A high sensitivity assay for the inflammatory marker C-Reactive protein employing acoustic biosensing. Journal of Nanobiotechnology, 6 5: 134-8. doi:10.1186/1477-3155-6-5


Author McBride, Jeffrey D.
Cooper, Matthew A.
Title A high sensitivity assay for the inflammatory marker C-Reactive protein employing acoustic biosensing
Journal name Journal of Nanobiotechnology   Check publisher's open access policy
ISSN 1477-3155
Publication date 2008-04-29
Sub-type Article (original research)
DOI 10.1186/1477-3155-6-5
Open Access Status DOI
Volume 6
Issue 5
Start page 134
End page 8
Total pages 8
Place of publication London, United Kingdom
Publisher BioMed Central
Language eng
Subject 1004 Medical Biotechnology
Abstract C-Reactive Protein (CRP) is an acute phase reactant routinely used as a biomarker to assess either infection or inflammatory processes such as autoimmune diseases. CRP also has demonstrated utility as a predictive marker of future risk of cardiovascular disease. A new method of immunoassay for the detection of C-Reactive Protein has been developed using Resonant Acoustic Profiling™ (RAP™) with comparable sensitivity to a high sensitivity CRP ELISA (hsCRP) but with considerable time efficiency (12 minutes turnaround time to result). In one method, standard solutions of CRP (0 to 231 ng/mL) or diluted spiked horse serum sample are injected through two sensor channels of a RAP™ biosensor. One contains a surface with sheep antibody to CRP, the other a control surface containing purified Sheep IgG. At the end of a 5-minute injection the initial rate of change in resonant frequency was proportional to CRP concentration. The initial rates of a second sandwich step of anti-CRP binding were also proportional to the sample CRP concentration and provided a more sensitive method for quantification of CRP. The lower limit of detection for the direct assay and the homogenous sandwich assay were both 20 ng/mL whereas for the direct sandwich assay the lower limit was 3 ng/mL. In a step towards a rapid clinical assay, diluted horse blood spiked with human CRP was passed over one sensor channel whilst a reference standard solution at the borderline cardiovascular risk level was passed over the other. A semi-quantities ratio was thus obtained indicative of sample CRP status. Overall, the present study revealed that CRP concentrations in serum that might be expected in both normal and pathological conditions can be detected in a time-efficient, label-free immunoassay with RAP™ detection technology with determined CRP concentrations in close agreement with those determined using a commercially available high sensitivity ELISA.
Keyword Resonant acoustic profiling
C-Reactive Protein (CRP)
Immunoassay
ELISA
Biomarkers
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Institute for Molecular Bioscience - Publications
 
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Created: Fri, 20 Nov 2009, 23:49:19 EST by Christine Ouslinis on behalf of Institute for Molecular Bioscience