Maternal neoangiogenesis during pregnancy partly derives from fetal endothelial progenitor cells

Huu, Sau Nguyen, Oster, Michele, Uzan, Serge, Chareyre, Fabrice, Aractingi, Selim and Khosrotehrani, Kiarash (2007) Maternal neoangiogenesis during pregnancy partly derives from fetal endothelial progenitor cells. Proceedings of the National Academy of Sciences of the United States of America, 104 6: 1871-1876. doi:10.1073/pnas.0606490104


Author Huu, Sau Nguyen
Oster, Michele
Uzan, Serge
Chareyre, Fabrice
Aractingi, Selim
Khosrotehrani, Kiarash
Title Maternal neoangiogenesis during pregnancy partly derives from fetal endothelial progenitor cells
Journal name Proceedings of the National Academy of Sciences of the United States of America   Check publisher's open access policy
ISSN 0027-8424
1091-6490
Publication date 2007-02-01
Sub-type Article (original research)
DOI 10.1073/pnas.0606490104
Open Access Status Not Open Access
Volume 104
Issue 6
Start page 1871
End page 1876
Total pages 6
Place of publication Washington D.C.
Publisher National Academy of Sciences of the U.S.
Language eng
Subject 111402 Obstetrics and Gynaecology
111601 Cell Physiology
Abstract Fetal progenitor cells enter the maternal circulation during pregnancy and can persist for decades. We aimed to determine the role of these cells in tissue inflammation during pregnancy. WT female mice were mated to males transgenic for the EGFP (ubiquitous) or the luciferase gene controlled by the VEGF receptor 2 (VEGFR2; V-Luc) promoter. A contact hypersensitivity reaction was triggered during such pregnancies. Fetal cells were tracked by using real-time quantitative amplification of the transgene (real-time PCR), Y chromosome in situ hybridization (FISH), immunofluorescence or in vivo bioluminescence imaging. Real-time PCR disclosed fetal cells in the inflamed areas in all tested mice (17/17) with higher frequency and numbers in the inflamed compared with the control areas (P = 0.01). Double labeling demonstrated CD31+ EGFP+ fetal cells organized as blood vessels. In WT pregnant mice bearing V-Luc fetuses, a specific luciferase activity signal could be detected at the hypersensitivity site only, demonstrating the elective presence of VEGFR2-expressing fetal cells. In conclusion, using various techniques, we found the presence of fetal endothelial cells lining blood vessels in maternal sites of inflammation. These results imply that fetal endothelial progenitor cells are acquired by the mother and participate in maternal angiogenesis during pregnancy.
Keyword Angiogenesis factor
Chimerism
Gestation
Fetal stem cells
Noninvasive prenatal diagnosis
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
UQ Centre for Clinical Research Publications
 
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Created: Tue, 17 Nov 2009, 22:26:57 EST