Ecology of uncultivated Oscillospira species in the rumen of cattle, sheep, and reindeer as assessed by microscopy and molecular approaches

Mackie, Roderick I., Aminov, Rustam I., Hu, Wenping, Klieve, Athol V., Ouwerkerk, Diane, Sundset, Monica A. and Kamagata, Yoichi (2003) Ecology of uncultivated Oscillospira species in the rumen of cattle, sheep, and reindeer as assessed by microscopy and molecular approaches. Applied and Environmental Microbiology, 69 11: 6808-6815. doi:10.1128/AEM.69.11.6808-6815.2003

Attached Files (Some files may be inaccessible until you login with your UQ eSpace credentials)
Name Description MIMEType Size Downloads
UQ186492_OA.pdf Full text (open access) application/pdf 1.08MB 0

Author Mackie, Roderick I.
Aminov, Rustam I.
Hu, Wenping
Klieve, Athol V.
Ouwerkerk, Diane
Sundset, Monica A.
Kamagata, Yoichi
Title Ecology of uncultivated Oscillospira species in the rumen of cattle, sheep, and reindeer as assessed by microscopy and molecular approaches
Journal name Applied and Environmental Microbiology   Check publisher's open access policy
ISSN 0099-2240
1098-5336
Publication date 2003-11-01
Year available 2003
Sub-type Article (original research)
DOI 10.1128/AEM.69.11.6808-6815.2003
Open Access Status File (Publisher version)
Volume 69
Issue 11
Start page 6808
End page 6815
Total pages 8
Place of publication Washington, D.C.
Publisher American Society for Microbiology
Language eng
Subject 0605 Microbiology
07 Agricultural and Veterinary Sciences
Abstract The ecology of the uncultured, but large and morphologically conspicuous, rumen bacterium Oscillospira spp. was studied. Oscillospira-specific 16S rRNA gene sequences were detected in North American domestic cattle, sheep from Australia and Japan, and Norwegian reindeer. Phylogenetic analysis of the sequences obtained allowed definition of three operational taxonomic units within the Oscillospira clade. Consistent with this genetic diversity, we observed atypical smaller morphotypes by using an Oscillospira-specific fluorescence in situ hybridization probe. Despite the visual disappearance of typical large Oscillospira morphotypes, the presence of Oscillospira spp. was still detected by Oscillospira-specific PCR in the rumen of cattle and sheep. These observations suggest the broad presence of Oscillospira species in various rumen ecosystems with the level, and most likely the morphological form, dependent on diet. An ecological analysis based on enumeration of the morphologically conspicuous, large-septate form confirms that the highest counts are associated with the feeding of fresh forage diets to cattle and sheep and in two different subspecies of reindeer investigated.
Keyword 16s Ribosomal-rna
In-situ Detection
Phylogenetic Analysis
Sp-nov
Sequence-analysis
Diurnal Changes
Gen-nov
Organisms
Bacteria
Guillermondii
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Agriculture and Food Sciences
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 61 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 64 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 17 Nov 2009, 22:02:00 EST