Conjugative plasmid transfer and adhesion dynamics in an Escherichia coli Biofilm

Ong, Cheryl-Lynn Y., Beatson, Scott A., McEwan, Alastair G. and Schembri, Mark A. (2009) Conjugative plasmid transfer and adhesion dynamics in an Escherichia coli Biofilm. Applied and Environmental Microbiology, 75 21: 6783-6791. doi:10.1128/AEM.00974-09

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Author Ong, Cheryl-Lynn Y.
Beatson, Scott A.
McEwan, Alastair G.
Schembri, Mark A.
Title Conjugative plasmid transfer and adhesion dynamics in an Escherichia coli Biofilm
Formatted title
Conjugative plasmid transfer and adhesion dynamics in an Escherichia coli biofilm
Journal name Applied and Environmental Microbiology   Check publisher's open access policy
ISSN 0099-2240
Publication date 2009-08-28
Year available 2009
Sub-type Article (original research)
DOI 10.1128/AEM.00974-09
Open Access Status File (Publisher version)
Volume 75
Issue 21
Start page 6783
End page 6791
Total pages 9
Editor L Nicholson Ornston
Place of publication United States
Publisher American Society for Microbiology
Language eng
Subject C1
060501 Bacteriology
060502 Infectious Agents
060503 Microbial Genetics
970106 Expanding Knowledge in the Biological Sciences
920109 Infectious Diseases
Abstract A conjugative plasmid from the catheter-associated urinary tract infection strain Escherichia coli MS2027 was sequenced and annotated. This 42,644-bp plasmid, designated pMAS2027, contains 58 putative genes and is most closely related to plasmids belonging to incompatibility group X (IncX1). Plasmid pMAS2027 encodes two important virulence factors: type 3 fimbriae and a type IV secretion (T4S) system. Type 3 fimbriae, recently found to be functionally expressed in E. coli, played an important role in biofilm formation. Biofilm formation by E. coli MS2027 was specifically due to expression of type 3 fimbriae and not the T4S system. The T4S system, however, accounted for the conjugative ability of pMAS2027 and enabled a non-biofilm-forming strain to grow as part of a mixed biofilm following acquisition of this plasmid. Thus, the importance of conjugation as a mechanism to spread biofilm determinants was demonstrated. Conjugation may represent an important mechanism by which type 3 fimbria genes are transferred among the Enterobacteriaceae that cause device-related infections in nosocomial settings.
Keyword Biotechnology & Applied Microbiology
Microbiology
Biotechnology & Applied Microbiology
Microbiology
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
MICROBIOLOGY
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID DP666852
455914
Institutional Status UQ
Additional Notes Supplemental material (read only) - http://aem.asm.org/content/75/21/6783/suppl/DC1

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
School of Chemistry and Molecular Biosciences
 
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Created: Thu, 12 Nov 2009, 21:55:28 EST by Mr Andrew Martlew on behalf of School of Chemistry & Molecular Biosciences