Differentiation of human fetal mesenchymal stem cells into cells with an oligodendrocyte phenotype.

Kennea, Nigel L., Waddington, Simon, N., Chan, Jerry, O'Donoghue, Keelin, Yeung, Davy, Taylor, Deanna L., Al-Allaf, Faisal A., Pirianov, Grisha, Themis, Michael, Edwards, A. David, Fisk, Nicholas M. and Mehmet, Huseyin (2009) Differentiation of human fetal mesenchymal stem cells into cells with an oligodendrocyte phenotype.. Cell Cycle, 8 7: 1069-1079. doi:10.4161/cc.8.7.8121

Author Kennea, Nigel L.
Waddington, Simon, N.
Chan, Jerry
O'Donoghue, Keelin
Yeung, Davy
Taylor, Deanna L.
Al-Allaf, Faisal A.
Pirianov, Grisha
Themis, Michael
Edwards, A. David
Fisk, Nicholas M.
Mehmet, Huseyin
Title Differentiation of human fetal mesenchymal stem cells into cells with an oligodendrocyte phenotype.
Journal name Cell Cycle   Check publisher's open access policy
ISSN 1538-4101
Publication date 2009-04-01
Year available 2009
Sub-type Article (original research)
DOI 10.4161/cc.8.7.8121
Volume 8
Issue 7
Start page 1069
End page 1079
Total pages 11
Editor Mikhail V Blagosklonny
Place of publication Austin, TX
Publisher Landes Bioscience
Language eng
Subject C1
100404 Regenerative Medicine (incl. Stem Cells and Tissue Engineering)
Abstract The potential of mesenchymal stem cells (MSC) to differentiate into neural lineages has raised the possibility of autologous cell transplantation as a therapy for neurodegenerative diseases. We have identified a population of circulating human fetal mesenchymal stem cells (hfMSC) that are highly proliferative and can readily differentiate into mesodermal lineages such as bone, cartilage, fat and muscle. Here, we demonstrate for the first time that primary hfMSC can differentiate into cells with an oligodendrocyte phenotype both in vitro and in vivo. By exposing hfMSC to neuronal conditioned medium or by introducing the pro-oligodendrocyte gene, Olig-2, hfMSC adopted an oligodendrocyte-like morphology, expressed oligodendrocyte markers and appeared to mature appropriately in culture. Importantly we also demonstrate the differentiation of a clonal population of hfMSC into both mesodermal (bone) and ectodermal (oligodendrocyte) lineages. In the developing murine brain transplanted hfMSC integrated into the parenchyma but oligodendrocyte differentiation of these naïve hfMSC was very low. However, the proportion of cells expressing oligodendrocyte markers increased significantly (from 0.2% to 4%) by preexposing the cells to differentiation medium in vitro prior to transplantation. Importantly, the process of in vivo differentiation occurred without cell fusion. These findings suggest that hfMSC may provide a potential source of oligodendrocytes for study and potential therapy.
Keyword Oligodendrocyte
Q-Index Code C1
Q-Index Status Provisional Code

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
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Created: Mon, 12 Oct 2009, 21:07:22 EST by Carmen Buttery on behalf of UQ Centre for Clinical Research