Identification of a novel calcitonin receptor response element in the promoter of the human p21WAF1/CIP1 gene

Evdokiou, A., Raggatt, L. J., Sakai, T. and Findlay, M. D. (2000) Identification of a novel calcitonin receptor response element in the promoter of the human p21WAF1/CIP1 gene. Journal Of Molecular Endocrinology, 25 2: 195-206. doi:10.1677/jme.0.0250195


Author Evdokiou, A.
Raggatt, L. J.
Sakai, T.
Findlay, M. D.
Title Identification of a novel calcitonin receptor response element in the promoter of the human p21WAF1/CIP1 gene
Journal name Journal Of Molecular Endocrinology   Check publisher's open access policy
ISSN 1479-6813
0952-5041
Publication date 2000-10-01
Sub-type Article (original research)
DOI 10.1677/jme.0.0250195
Volume 25
Issue 2
Start page 195
End page 206
Total pages 12
Place of publication Bristol, England
Publisher Society for Endocrinology
Language eng
Subject 110314 Orthopaedics
110306 Endocrinology
Abstract The cyclin-dependent kinase inhibitor p21/WAF1/CIP1 is induced in many cell types in response to a variety of extracellular signals and causes cell cycle arrest in both the G1 and G2/M phases of the cell cycle. We reported previously that calcitonin (CT) receptor (CTR)-mediated growth inhibition of HEK-293 cells stably transfected with the human CTR is accompanied by a rapid and sustained induction of p21 and cell cycle arrest in G2. In the present study we have shown that CT stimulates transcription from a p21 promoter-luciferase construct. Using deletion and mutation analysis of the p21 promoter we have demonstrated that transcriptional activation of p21 by CT is p53-independent and is mediated through specific activation of Sp1-binding sites in a region of the promoter between -82 and -69, relative to the transcription start site. CTR-mediated transcriptional activation of p21 was specific for the insert-negative isoform of the human CTR. Butyrate, which was shown previously to activate the same Sp1 site, synergised with CT to increase further p21 promoter activity. These results provide the first demonstration that CTR can induce gene transcription through the constitutively expressed transcription factor Sp1, and define a mechanism of cell growth suppression that may have implications for other members of the seven-transmembrane domain G protein-coupled receptor superfamily.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Molecular Bioscience - Publications
 
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