The Gal4/UAS toolbox in zebrafish: new approaches for defining behavioral circuits

Scott, E. K. (2009) The Gal4/UAS toolbox in zebrafish: new approaches for defining behavioral circuits. Journal Of Neurochemistry, 110 2: 441-456. doi:10.1111/j.1471-4159.2009.06161.x

Author Scott, E. K.
Title The Gal4/UAS toolbox in zebrafish: new approaches for defining behavioral circuits
Journal name Journal Of Neurochemistry   Check publisher's open access policy
ISSN 0022-3042
Publication date 2009-07-01
Year available 2009
Sub-type Article (original research)
DOI 10.1111/j.1471-4159.2009.06161.x
Volume 110
Issue 2
Start page 441
End page 456
Total pages 16
Editor Sean Murphy
Tony J Turner
Place of publication United Kingdom
Publisher Wiley-Blackwell Publishing Ltd.
Language eng
Subject C1
920114 Reproductive System and Disorders
110902 Cellular Nervous System
060801 Animal Behaviour
Abstract Over recent years, several groundbreaking techniques have been developed that allow for the anatomical description of neurons, and the observation and manipulation of their activity. Combined, these approaches should provide a great leap forward in our understanding of the structure and connectivity of the nervous system and how, as a network of individual neurons, it generates behavior. Zebrafish, given their external development and optical transparency, are an appealing system in which to employ these methods. These traits allow for direct observation of fluorescence in describing anatomy and observing neural activity, and for the manipulation of neurons using a host of light-triggered proteins. Gal4/Upstream Activating Sequence techniques, as they are based on a binary system, allow for the flexible deployment of a range of transgenes in expression patterns of interest. As such, they provide a promising approach for viewing neurons in a variety of ways, each of which can reveal something different about their structure, connectivity, or function. In this study, the author will review recent progress in the development of the Gal4/Upstream Activating Sequence system in zebrafish, feature examples of promising studies to date, and examine how various new technologies can be used in the future to untangle the complex mechanisms by which behavior is generated.
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
School of Biomedical Sciences Publications
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Citation counts: TR Web of Science Citation Count  Cited 35 times in Thomson Reuters Web of Science Article | Citations
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Created: Thu, 10 Sep 2009, 22:10:44 EST by Cameron Harris on behalf of School of Biomedical Sciences