Proteomic analysis of bovine conceptus fluids during early pregnancy

Riding, George A., Jones, Alun, Holland, Michael K., Hill, Jonathan R. and Lehnert, Sigrid A. (2008) Proteomic analysis of bovine conceptus fluids during early pregnancy. Proteomics, 8 1: 160-177. doi:10.1002/pmic.200700465


Author Riding, George A.
Jones, Alun
Holland, Michael K.
Hill, Jonathan R.
Lehnert, Sigrid A.
Title Proteomic analysis of bovine conceptus fluids during early pregnancy
Journal name Proteomics   Check publisher's open access policy
ISSN 1615-9853
Publication date 2008-01-01
Year available 2008
Sub-type Article (original research)
DOI 10.1002/pmic.200700465
Open Access Status Not yet assessed
Volume 8
Issue 1
Start page 160
End page 177
Total pages 18
Place of publication Germany
Publisher Wiley - V C H Verlag GmbH & Co. KGaA
Language eng
Abstract A proteomic analysis of bovine amniotic and allantoic fluids collected around Day 45 of gestation was performed using gel-based and LC-based MS workflows. A depletion/enrichment protocol using ultrafiltration under denaturing and reducing conditions produced an enriched fraction containing protein species predominantly between 5 and 50 kDa molecular weight. The analyses of conceptus fluid proteins were performed using two strategies; first, 2-DE coupled with MALDI-TOF-MS/MS and LC-ESI-MS/MS analysis of individual protein spots and second, a global protein snapshot of the enriched 5-50 kDa protein fraction by LC-ESI-MS/MS and LC-MALDI-TOF-MS/MS. Allocation of bovine specific protein identities was achieved by searching the Interactive Bovine In Silico SNP (IBISS) and NCBInr protein sequence databases resulting in the confident PMF identification and MS/MS confirmation of >200 2-DE generated allantoic fluids protein spots (74 individual protein species identified) and the MS/MS peptide identification of 105 LC-ESI-MS/MS generated protein identities. In total, the identity of 139 individual protein species from allantoic fluids was confirmed with peptide sequence probability MOWSE scores at the p<0.05 level or better. The comparison of bovine Day 45 amniotic and allantoic fluids protein profiles revealed differences between these two conceptus fluids in early pregnancy.
Keyword Allantoic Fluid
2-dimensional Gel-electrophoresis
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Molecular Bioscience - Publications
 
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Created: Thu, 03 Sep 2009, 20:31:47 EST by Mr Andrew Martlew on behalf of Institute for Molecular Bioscience