Nanoparticle-delivered biosensor for reactive oxygen species in diabetes

Prow, Tarl W., Bhutto, Imran, Grebe, Rhonda, Uno, Koichi, Merges, Carol, Mcleod, D. Scott and Lutty, Gerard A. (2008) Nanoparticle-delivered biosensor for reactive oxygen species in diabetes. Vision Research, 48 3: 478-485. doi:10.1016/j.visres.2007.09.019

Author Prow, Tarl W.
Bhutto, Imran
Grebe, Rhonda
Uno, Koichi
Merges, Carol
Mcleod, D. Scott
Lutty, Gerard A.
Title Nanoparticle-delivered biosensor for reactive oxygen species in diabetes
Journal name Vision Research   Check publisher's open access policy
ISSN 0042-6989
Publication date 2008-02-01
Year available 2008
Sub-type Article (original research)
DOI 10.1016/j.visres.2007.09.019
Open Access Status DOI
Volume 48
Issue 3
Start page 478
End page 485
Total pages 8
Editor D. M. Levi
Place of publication East Park, Kidlington, Oxford, U.K.
Publisher Pergamon
Language eng
Subject 100709 Nanomedicine
1113 Ophthalmology and Optometry
Formatted abstract
The cell’s own antioxidant response element (ARE) can be used to evaluate the complications of diabetes mellitus. The hypothesis that a synthetic ARE could be used as a genetic switch, or biosensor, to turn on and off therapeutic genes is tested herein. Mitochondrial oxidative stress (MOS) has been hypothesized as one of the earliest insults in diabetes. Fluorescent probes used to monitor MOS revealed that the addition of glucose at physiological levels to cultures of endothelial cells was able to induce MOS above normal levels and in a dose-dependant manner. Additional data showed that increased glucose levels activated the ARE-GFP in a dose-dependant manner. These data support the hypothesis that the induction of MOS is more sensitive to hyperglycemia than the induction of the ARE. Delivery of an ARE-GFP construct with nanoparticles to the eye was successful using sub-retinal injection. This ARE-GFP/nanoparticle construct was functional and reported the activation of the ARE in diabetic rat retinal pigment epithelium (RPE). These data support the use of nanoparticle-delivered biosensors for monitoring the oxidative status of tissues in vivo.
Keyword Diabetes
Antioxidant response element
Gene delivery
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID R03 EY013744-03
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Australian Institute for Bioengineering and Nanotechnology Publications
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Citation counts: TR Web of Science Citation Count  Cited 13 times in Thomson Reuters Web of Science Article | Citations
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Created: Thu, 03 Sep 2009, 20:21:38 EST by Mr Andrew Martlew on behalf of Medicine - Princess Alexandra Hospital