Expression and putative roles in attachment of outer membrane proteins of Escherichia coli O157 from planktonic and sessile culture

Rivas, Lucia, Fegan, Narelle and Dykes, Gary A. (2008) Expression and putative roles in attachment of outer membrane proteins of Escherichia coli O157 from planktonic and sessile culture. Foodborne Pathogens and Disease, 5 2: 155-164. doi:10.1089/fpd.2007.0052


Author Rivas, Lucia
Fegan, Narelle
Dykes, Gary A.
Title Expression and putative roles in attachment of outer membrane proteins of Escherichia coli O157 from planktonic and sessile culture
Formatted title
Expression and putative roles in attachment of outer membrane proteins of Escherichia coli O157 from planktonic and sessile culture
Journal name Foodborne Pathogens and Disease   Check publisher's open access policy
ISSN 1535-3141
1556-7125
Publication date 2008-04-13
Sub-type Article (original research)
DOI 10.1089/fpd.2007.0052
Open Access Status Not Open Access
Volume 5
Issue 2
Start page 155
End page 164
Total pages 10
Editor Stephen P. Oliver
Place of publication New York, United States
Publisher Mary Ann Liebert
Language eng
Formatted abstract
Many strains of Shiga toxigenic Escherichia coli (STEC), particularly the serotype O157:H7, are foodborne pathogens causing disease in many countries throughout the world. E. coli O157:H7 is able to attach and survive on various surfaces such as stainless steel (SS) found within the food processing environment. We examined the outer membrane protein (OMP) profiles of four E. coli O157 (three toxigenic O157:H7 and one nontoxigenic O157:HR) and one non-STEC strain (O1:H7), previously reported to have different abilities to attach to SS following growth in planktonic (nutrient broth) and sessile (nutrient agar) culture. The OMPs of the five E. coli strains grown in planktonic and sessile culture were extracted using N-lauroyl sarcosine and the OMP profiles were separated using two-dimensional (2D) gel electrophoresis. Qualitative and quantitative variations in the total number of OMPs expressed between planktonic and sessile cultures were found for all E. coli isolates tested. A number of differentially expressed protein spots were selected from 2D gels and were identified. FlgE was found to be expressed in planktonic culture but not sessile culture. MipA and OmpX had higher expression in sessile culture than planktonic culture, while expression of OmpA did not differ between E. coli strains or between the two modes of growth. Although differential expression of OMPs was found between isolates grown in planktonic and sessile culture, further investigations are required to determine a role of some of these identified proteins during growth of E. coli in planktonic and sessile culture and their influence during the attachment process.
Keyword Epithelial-cells
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Agriculture and Food Sciences
 
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Created: Thu, 03 Sep 2009, 20:08:52 EST by Mr Andrew Martlew on behalf of School of Land, Crop and Food Sciences