A novel rat model for glioblastoma multiforme using a bioluminescent F98 cell line

Bryant, M. J., Chuah,T. L., Luff, J., Lavin, M. F. and Walker, D. G. (2008) A novel rat model for glioblastoma multiforme using a bioluminescent F98 cell line. Journal of Clinical Neuroscience, 15 5: 545-551. doi:10.1016/j.jocn.2007.04.022


Author Bryant, M. J.
Chuah,T. L.
Luff, J.
Lavin, M. F.
Walker, D. G.
Title A novel rat model for glioblastoma multiforme using a bioluminescent F98 cell line
Journal name Journal of Clinical Neuroscience   Check publisher's open access policy
ISSN 0967-5868
1532-2653
Publication date 2008-05-01
Sub-type Article (original research)
DOI 10.1016/j.jocn.2007.04.022
Volume 15
Issue 5
Start page 545
End page 551
Total pages 7
Place of publication South Melbourne, Victoria
Publisher Churchill Livingstone
Language eng
Subject 1112 Oncology and Carcinogenesis
Abstract In experimental neuro-oncology there remains a need for animal models that can be used to assess the efficacy of new and innovative treatment methodologies for glioblastoma multiforme (GBM). Rat models have remained the mainstay of neuro-oncology research for over 30 years; however, despite extensive experimentation, there is no one rat model that truly reflects the features of human tumours. We have developed a novel rat brain tumour model that closely resembles human GBM in biological behaviour and that utilizes bioluminescence imaging (BLI) to follow day-to-day in vivo progress of the tumour. F98 glioma cells were transfected with the firefly luciferase gene and injected orthotopically into the brains of 24 rats. Weekly BLI after subcutaneous injection of luciferin allowed for in vivo monitoring of the progress of the brain tumours. Euthanasia and histological analysis of the rodent brains at varying stages post-implantation, allowed for statistically significant correlation between tumour size and luminescence (p = 0.002). The utility of this model is readily apparent, allowing us a way of examining the effects of new and novel therapeutics in these rats.
Keyword Bioluminescence imaging
Rodent model
F98 glioma
Luciferase
Q-Index Code C1
Q-Index Status Provisional Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
UQ Centre for Clinical Research Publications
 
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Created: Thu, 03 Sep 2009, 20:08:20 EST by Mr Andrew Martlew on behalf of Faculty Of Health Sciences