Differential proteomic analysis of bovine conceptus fluid proteins in pregnancies generated by assisted reproductive technologies

Riding, George A., Hill, Jonathan R., Jones, Alun, Holland, Michael K., Josh, Peter F. and Lehnert, Sigrid A. (2008) Differential proteomic analysis of bovine conceptus fluid proteins in pregnancies generated by assisted reproductive technologies. Protemics, 8 14: 2967-2982. doi:10.1002/pmic.200800008


Author Riding, George A.
Hill, Jonathan R.
Jones, Alun
Holland, Michael K.
Josh, Peter F.
Lehnert, Sigrid A.
Title Differential proteomic analysis of bovine conceptus fluid proteins in pregnancies generated by assisted reproductive technologies
Journal name Protemics   Check publisher's open access policy
ISSN 1615-9853
Publication date 2008-07-01
Year available 2008
Sub-type Article (original research)
DOI 10.1002/pmic.200800008
Open Access Status Not yet assessed
Volume 8
Issue 14
Start page 2967
End page 2982
Total pages 16
Place of publication Germany
Publisher Wiley - V C H Verlag GmbH & Co. KGaA
Language eng
Abstract Proteomic analysis of bovine conceptus fluid proteins during early pregnancy has the potential to expose protein species indicative of both the overall health of the fetal-maternal environment and fetal developmental status. In this study, we examined the differential abundance of bovine conceptus fluid proteins (5-50 kDa fraction) from-naturally conceived, in vitro fertilisation (IVF) and somatic cell nuclear transfer (SCNT)-derived pregnancies at days 45 and 90 of gestation. In day 45 allantoic fluid (A11F) samples, an atypical cluster of low molecular weight (similar to 14-16 kDa), low pI (between 3.0 and 4.5 pH units) protein species was increased in three of four IVF samples (30-100-fold increase in protein spot volumes compared to normal). These proteins were identified as paralogs of the bovine cathelicidin antimicrobial protein (CAMP) by MALDI-TOF MS peptide mass fingerprint and MALDI-TOF MS/MS peptide sequence analysis. Peptidoglycan recognition protein and serine (or cysteine) proteinase inhibitor clade B1, were also significantly increased in the corresponding IVF samples. In two of four SCNTA11F samples, a 2-10-fold increase in CAMP protein spot volumes were detected. No aberrant abundance levels of individual protein species were observed in amniotic fluid samples, or in day 90 IVF A11F samples. Identification of unique protein species present in the normal bovine A11F proteome at day 45 is also reported.
Formatted abstract
Proteomic analysis of bovine conceptus fluid proteins during early pregnancy has the potential to expose protein species indicative of both the overall health of the fetal-maternal environment and fetal developmental status. In this study, we examined the differential abundance of bovine conceptus fluid proteins (5-50 kDa fraction) from naturally conceived, in vitro fertilisation (IVF) and somatic cell nuclear transfer (SCNT)-derived pregnancies at days 45 and 90 of gestation. In day 45 allantoic fluid (AllF) samples, an atypical cluster of low molecular weight (∼14-16 kDa), low pI (between 3.0 and 4.5 pH units) protein species was increased in three of four IVF samples (30-100-fold increase in protein spot volumes compared to normal). These proteins were identified as paralogs of the bovine cathelicidin antimicrobial protein (CAMP) by MALDI-TOF MS peptide mass fingerprint and MALDI-TOF MS/MS peptide sequence analysis. Peptidoglycan recognition protein and serine (or cysteine) proteinase inhibitor clade B1, were also significantly increased in the corresponding IVF samples. In two of four SCNT AllF samples, a 2-10-fold increase in CAMP protein spot volumes were detected. No aberrant abundance levels of individual protein species were observed in amniotic fluid samples, or in day 90 IVF AllF samples. Identification of unique protein species present in the normal bovine AllF proteome at day 45 is also reported. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.
Keyword Allantoic Fluid
In-vitro
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Molecular Bioscience - Publications
 
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Created: Thu, 03 Sep 2009, 19:44:36 EST by Mr Andrew Martlew on behalf of Institute for Molecular Bioscience