BioPEGylation of Polyhydroxyalkanoates: Influence on Properties and Satellite-Stem Cell Cycle

Marcal, Helder, Wanandy, Nico S., Sanguanchaipaiwong, Vorapat, Woolnough, Catherine E., Lauto, Antonio, Mahler, Stephen M. and Foster, L. John R. (2008) BioPEGylation of Polyhydroxyalkanoates: Influence on Properties and Satellite-Stem Cell Cycle. Biomacromolecules, 9 10: 2719-2726. doi:10.1021/bm800418e

Author Marcal, Helder
Wanandy, Nico S.
Sanguanchaipaiwong, Vorapat
Woolnough, Catherine E.
Lauto, Antonio
Mahler, Stephen M.
Foster, L. John R.
Title BioPEGylation of Polyhydroxyalkanoates: Influence on Properties and Satellite-Stem Cell Cycle
Journal name Biomacromolecules   Check publisher's open access policy
ISSN 1525-7797
Publication date 2008-10-01
Year available 2008
Sub-type Article (original research)
DOI 10.1021/bm800418e
Open Access Status
Volume 9
Issue 10
Start page 2719
End page 2726
Total pages 8
Place of publication United States
Publisher American Chemical Society
Language eng
Abstract The addition of poly(ethylene glycol), PEG, to bioprocessing systems producing polyhydroxyalkanoates (PHAs), has been reported as a means of their molecular weight control and can also support bioPEGylation, resulting in hybrids with amphiphillic properties. However, the study of such natural-synthetic hybrids of PHA-b-PEG is still in its infancy. In this study, we report the influence of bioPEGylation of polyhydroxyoctanoate (PHO) on its physiochemical, material, and biological properties. Consistent with previous studies, bioPEGylation with diethylene glycol (DEG) showed a significant reduction in PHA molecular weight (57%). In comparison to solvent cast films of PHO, PHO-b-DEG films possessed a noticeable X-ray diffraction peak at 9.82° and increased Young's modulus of 11 Gpa (83%). Potential biocompatibility was investigated by measuring the early phase of apoptosis in myoblastic satellite-stem cells (C2C12). Comparative analysis of cell proliferation and progression in the presence of the mcl-PHA and its hybrid showed that the latter induced significant cell cycle progression: the first time a biomaterial has been shown to do so. Microtopographies of the film surfaces demonstrated that these differences were not due to changes in surface morphology; both polymers possessed average surface rugosities of 1.4 ± 0.2 μm. However, a slight decrease in surface hydrophobicity (3.5 ± 0.9°) due to the hydrophilic DEG may have exerted an influence. The results support the further study of bioPEGylated PHAs as potential biomaterials in the field of tissue engineering.
Keyword Polyhydroxyoctanoate-Diethylene Glycol
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
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Citation counts: TR Web of Science Citation Count  Cited 13 times in Thomson Reuters Web of Science Article | Citations
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Created: Thu, 03 Sep 2009, 19:23:36 EST by Mr Andrew Martlew on behalf of Aust Institute for Bioengineering & Nanotechnology