Rapid identification of Aedes albopictus, Aedes scutellaris, and Aedes aegypti life stages using real-time polymerase chain reaction assays

Hill, Lydia A., Davis, Joseph B., Hapgood, George, Whelan, Peter I., Smith, Greg A., Ritchie, Scott A., Cooper, R. D. and van den Hurk, Andrew F. (2008) Rapid identification of Aedes albopictus, Aedes scutellaris, and Aedes aegypti life stages using real-time polymerase chain reaction assays. American Journal of Tropical Medicine and Hygiene, 79 6: 866-875.

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Author Hill, Lydia A.
Davis, Joseph B.
Hapgood, George
Whelan, Peter I.
Smith, Greg A.
Ritchie, Scott A.
Cooper, R. D.
van den Hurk, Andrew F.
Title Rapid identification of Aedes albopictus, Aedes scutellaris, and Aedes aegypti life stages using real-time polymerase chain reaction assays
Journal name American Journal of Tropical Medicine and Hygiene   Check publisher's open access policy
ISSN 0002-9637
1476-1645
Publication date 2008-12-01
Sub-type Article (original research)
Volume 79
Issue 6
Start page 866
End page 875
Total pages 10
Place of publication Deerfield, IL, United States
Publisher American Society of Tropical Medicine and Hygiene
Language eng
Formatted abstract
In 2005, a widespread infestation of Aedes albopictus was discovered in the Torres Strait, the region between northern Australia and New Guinea. To contain this species, an eradication program was implemented in 2006. However, the progress of this program is impeded by the difficulty of morphologically separating Ae. albopictus larvae from the endemic species Aedes scutellaris. In this study, three real-time TaqMan polymerase chain reaction assays that target the ribosomal internal transcribed spacer 1 region were developed to rapidly identify Aedes aegypti, Ae. albopictus, and Ae. scutellaris from northern Australia. Individual eggs, larvae, pupae, and adults, as well as the species composition of mixed pools were accurately identified. The assay method was validated using 703 field-collected specimens from the Torres Strait.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Molecular Bioscience - Publications
 
Citation counts: TR Web of Science Citation Count  Cited 20 times in Thomson Reuters Web of Science Article | Citations
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Created: Thu, 03 Sep 2009, 19:11:05 EST by Mr Andrew Martlew on behalf of Institute for Molecular Bioscience