Evaluation of growth performance in a diallel cross of three strains of giant freshwater prawn (Macrobrachium rosenbergii) in Vietnam

Thanh, NM, Ponzoni, RW, Nguyen, NH, Vu, NT, Barnes, A and Mather, PB (2009) Evaluation of growth performance in a diallel cross of three strains of giant freshwater prawn (Macrobrachium rosenbergii) in Vietnam. AQUACULTURE, 287 1-2: 75-83. doi:10.1016/j.aquaculture.2008.10.051

Author Thanh, NM
Ponzoni, RW
Nguyen, NH
Vu, NT
Barnes, A
Mather, PB
Title Evaluation of growth performance in a diallel cross of three strains of giant freshwater prawn (Macrobrachium rosenbergii) in Vietnam
Formatted title
Evaluation of growth performance in a diallel cross of three strains of giant freshwater prawn (Macrobrachium rosenbergii) in Vietnam
Journal name AQUACULTURE   Check publisher's open access policy
ISSN 0044-8486
Publication date 2009-02-01
Sub-type Article (original research)
DOI 10.1016/j.aquaculture.2008.10.051
Open Access Status DOI
Volume 287
Issue 1-2
Start page 75
End page 83
Total pages 9
Editor Barbara E. Brown
Place of publication Netherlands
Publisher Elsevier BV
Language eng
Subject C1
830101 Aquaculture Crustaceans (excl. Rock Lobster and Prawns)
070401 Aquaculture
Abstract In decapod crustaceans, the antennal gland (AnG) is a major primary source of externally secreted biomolecules, and some may act as pheromones that play a major role in aquatic animal communication. In aquatic crustaceans, sex pheromones regulate reproductive behaviours, yet they remain largely unidentified besides the N-acetylglucosamine-1,5-lactone (NAGL) that stimulates male to female attraction. In this study, we used an AnG transcriptome of the female giant freshwater prawn (Macrobrachium rosenbergii) to predict the secretion of 226 proteins, including the most abundantly expressed transcripts encoding the Spaetzle protein, a serine protease inhibitor, and an arthropodial cuticle protein AMP 8.1. A quantitative proteome analysis of the female AnG at intermolt, premolt and postmolt, identified numerous proteins of different abundances, such as the hemocyanin subunit 1 that is most abundant at intermolt. We also show that hemocyanin subunit 1 is present within water surrounding females. Of those metabolites identified, we demonstrate that the NAGL and N-acetylglucosamine (NAG) can bind with high affinity to hemocyanin subunit 1. In summary, this study has revealed components of the female giant freshwater prawn AnG that are released and contribute to further research towards understanding crustacean conspecific signalling.
Formatted abstract
The giant freshwater prawn (Macrobrachium rosenbergii) is one of the most important crustacean species
produced in inland aquaculture in many tropical and subtropical countries worldwide. The aim of the current
study was to evaluate the growth performance of three strains of giant freshwater prawn that originated
from geographically separated locations in a complete (3×3) diallel cross as a starting point for a stock
improvement program for the industry in Vietnam. Crosses were established over two generations using two
wild Vietnamese river populations (Dong Nai and Mekong) domesticated for the study and an introduced
Hawaiian strain that had been in culture for many generations. Juveniles from nine strain combinations were
produced using single-pair matings. Results after 15 weeks of grow-out in hapas showed that growth
performance of the Hawaiian strainwas best among the purebred strains and that certain cross combinations
grew signi!cantly faster than purebred strains. Mean body weights of speci!c cross combinations with Dong
Nai or Mekong as dams and the introduced strain (Hawaiian) as sires were signi!cantly heavier than those of
purebred Dong Nai or Mekong strains. While males reached heavier mean weights than females, male
variation among the strains was obscured by social factors that produced different frequencies of male
morphotypes (blue claw, orange claw and small males). Results suggest presence of potentially valuable
heterosis and possible impact of the direction of cross. From a practical viewpoint this could be exploited
upon by either, including different forms of crossbreeding, or alternatively, by creating a composite
population for future selection. Potential problems and challenges encountered during the trials are

Keyword Macrobrachium rosenbergii
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Biological Sciences Publications
Centre for Marine Studies Publications
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Citation counts: TR Web of Science Citation Count  Cited 34 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 39 times in Scopus Article | Citations
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Created: Thu, 03 Sep 2009, 18:53:11 EST by Mr Andrew Martlew on behalf of Centre for Marine Studies