Molecular characterization and analysis of a truncated serotonin receptor gene expressed in neural and reproductive tissues of abalone

Panasophonkul, S, Apisawetakan, S, Cummins, S, York, P, Degnan, B, Hanna, P, Saitongdee, P, Sobhon, P and Sretarugsa, P (2009) Molecular characterization and analysis of a truncated serotonin receptor gene expressed in neural and reproductive tissues of abalone. HISTOCHEMISTRY AND CELL BIOLOGY, 131 5: 629-642. doi:10.1007/s00418-009-0555-7


Author Panasophonkul, S
Apisawetakan, S
Cummins, S
York, P
Degnan, B
Hanna, P
Saitongdee, P
Sobhon, P
Sretarugsa, P
Title Molecular characterization and analysis of a truncated serotonin receptor gene expressed in neural and reproductive tissues of abalone
Journal name HISTOCHEMISTRY AND CELL BIOLOGY   Check publisher's open access policy
ISSN 0948-6143
Publication date 2009-05-01
Sub-type Article (original research)
DOI 10.1007/s00418-009-0555-7
Open Access Status Not yet assessed
Volume 131
Issue 5
Start page 629
End page 642
Total pages 14
Editor D. Drenckhahn
J. Roth
Place of publication Germany
Publisher Springer
Language eng
Subject C1
060305 Evolution of Developmental Systems
830103 Aquaculture Molluscs (excl. Oysters)
Formatted abstract
In molluscs, the neurotransmitter serotonin
(5-HT) has been linked to a variety of biological roles
including gamete maturation and spawning. The possible
involvement of 5-HT in abalone gamete release was
demonstrated by a dose-dependent increase in Haliotis rubra
gonad contractile bioactivity following 5-HT stimulation.
Physiological functions associated with 5-HT, are mediated
through binding to 5-HT receptors. A cDNA encoding a
putative 5-HT receptor consisting of 359 amino acids was
isolated from the tropical abalone H. asinina, termed 5-HT1 ha.
The 5-HT1 ha shares G-protein-coupled receptor motifs with
metazoan 5-HT receptors, including predicted transmembrane
domains, active sites for protein kinase action, and
N-linked glycosylation sites. However, the third intracellular
loop of 5-HT1 ha is relatively short, and only six transmembrane
domains are predicted, implying a truncated
receptor. Phylogenetic analysis with known 5-HT receptor
genes suggests that 5-HT1 ha belongs to the type 1 5-HT
receptor family. Expression analysis by RT-PCR showed
that 5-HT1 ha mRNA was present in all tissues examined,
including the neural ganglia and gonad tissues. Immunocytochemistry
revealed the presence of 5-HT1 ha speciWcally
within the soma of neuronal cells located in the outer cortex
of both cerebral and pleuropedal ganglia. In ovarian and
testicular tissues, 5-HT1 ha immunoreactivity was observed
in epithelial cells of the outer capsule and connective tissue
of the trabeculae to which the gamete follicles adhere.
Whether this receptor transcript is translated to a functional
protein needs to be veriWed, but if so, it could play a role in
reproduction.
Keyword Serotonin receptor
MUSSEL DREISSENA-POLYMORPHA
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
School of Biological Sciences Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 8 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 8 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Thu, 03 Sep 2009, 18:21:53 EST by Mr Andrew Martlew on behalf of School of Biological Sciences